Rabbit Anti-Rat IgG H&L preadsorbed (ab7099)
Key features and details
- Rabbit Anti-Rat IgG H&L preadsorbed
- Host species: Rabbit
- Suitable for: IHC-Fr, ICC/IF, WB, IHC-P, IP, Immunodiffusion, Conjugation, ELISA
Related conjugates and formulations
Overview
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Product name
Rabbit Anti-Rat IgG H&L preadsorbed
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Rat -
Tested applications
Suitable for: IHC-Fr, ICC/IF, WB, IHC-P, IP, Immunodiffusion, Conjugation, ELISAmore details -
Minimal
cross-reactivity
Human more details -
Immunogen
Full length native Rat IgG (purified).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads. -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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ICC/IF |
1/15000 - 1/75000.
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|
WB |
1/30000 - 1/150000.
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|
IHC-P |
Use at an assay dependent concentration.
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|
IP |
Use at an assay dependent concentration.
|
|
Immunodiffusion |
Use at an assay dependent concentration.
|
|
Conjugation |
Use at an assay dependent concentration.
|
|
ELISA |
1/300000 - 1/1.4e+006.
|
Notes |
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IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
1/15000 - 1/75000. |
WB
1/30000 - 1/150000. |
IHC-P
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Immunodiffusion
Use at an assay dependent concentration. |
Conjugation
Use at an assay dependent concentration. |
ELISA
1/300000 - 1/1.4e+006. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (5)
ab7099 has been referenced in 5 publications.
- Huang W et al. Oncogenic microRNA-181d binding to OGT contributes to resistance of ovarian cancer cells to cisplatin. Cell Death Discov 7:379 (2021). PubMed: 34876558
- Xu TH et al. OGT-Mediated KEAP1 Glycosylation Accelerates NRF2 Degradation Leading to High Phosphate-Induced Vascular Calcification in Chronic Kidney Disease. Front Physiol 11:1092 (2020). PubMed: 33192538
- Zhang CW et al. Long non-coding RNA PVT1 knockdown suppresses fibroblast-like synoviocyte inflammation and induces apoptosis in rheumatoid arthritis through demethylation of sirt6. J Biol Eng 13:60 (2019). PubMed: 31303891
- Recek N et al. Microplasma Induced Cell Morphological Changes and Apoptosis of Ex Vivo Cultured Human Anterior Lens Epithelial Cells - Relevance to Capsular Opacification. PLoS One 11:e0165883 (2016). PubMed: 27832099
- Jakobs C et al. AIM2 Drives Joint Inflammation in a Self-DNA Triggered Model of Chronic Polyarthritis. PLoS One 10:e0131702 (2015). Mouse . PubMed: 26114879