Overview

  • Product name

    Rabbit Anti-Rat IgG H&L (Texas Red ®)
    See all IgG secondary antibodies
  • Host species

    Rabbit
  • Target species

    Rat
  • Tested applications

    Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
  • Immunogen

    Rat IgG whole molecule

  • Conjugation

    Texas Red ®. Ex: 596nm, Em: 620nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes

    Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 4.9 moles Texas Redä per mole of Rabbit IgG
  • Clonality

    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.

Images

  • Immunofluorescence analysis of mouse fibroblasts, stained with a rat anti-mouse Lamp1 primary antibody and detected with ab6732 at 1/100 dilution.

References

This product has been referenced in:

  • Leclaire MD  et al. Lipofuscin-dependent stimulation of microglial cells. Graefes Arch Clin Exp Ophthalmol N/A:N/A (2019). Read more (PubMed: 30693383) »
  • Sun Y  et al. Ex Vivo and in Vivo Effects of Isofagomine on Acid ß-Glucosidase Variants and Substrate Levels in Gaucher Disease. J Biol Chem 287:4275-87 (2012). ICC/IF . Read more (PubMed: 22167193) »
See all 3 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab6732.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up