Key features and details
- Rabbit Anti-Rat IgG H&L (Texas Red ®)
- Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
- Host species: Rabbit
- Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA
Product nameRabbit Anti-Rat IgG H&L (Texas Red ®)
See all IgG secondary antibodies
Tested applicationsSuitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
Rat IgG whole molecule
ConjugationTexas Red ®. Ex: 596nm, Em: 620nm
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notesTexas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 4.9 moles Texas Redä per mole of Rabbit IgG
Our Abpromise guarantee covers the use of ab6732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Immunomicroscopy||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent dilution.|
|ICC/IF||1/1000 - 1/5000.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ELISA||1/10000 - 1/50000.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab6732 has been referenced in 3 publications.
- Leclaire MD et al. Lipofuscin-dependent stimulation of microglial cells. Graefes Arch Clin Exp Ophthalmol N/A:N/A (2019). PubMed: 30693383
- Sun Y et al. Ex Vivo and in Vivo Effects of Isofagomine on Acid ß-Glucosidase Variants and Substrate Levels in Gaucher Disease. J Biol Chem 287:4275-87 (2012). ICC/IF . PubMed: 22167193
- Teranishi N et al. Identification of neovasculature using nestin in colorectal cancer. Int J Oncol 30:593-603 (2007). PubMed: 17273760