Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.50
    Constituents: 0.164% Sodium phosphate, 50% Glycerol, 0.58% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Specific antibodies were absorpted by incubation with Sepharose-bound rat immunoglobulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized rat immunoglobulins a minimum of 65% protein bound.
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Dot blot
  • Application notes
    Recommended dilution as secondary antibody in ELISA is 1:1000.
    Recommended dilution as secondary antibody in dot-blotting is 1:100.
  • References

    This product has been referenced in:
    • Lahoud MH  et al. Targeting antigen to mouse dendritic cells via Clec9A induces potent CD4 T cell responses biased toward a follicular helper phenotype. J Immunol 187:842-50 (2011). Read more (PubMed: 21677141) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for contacting us.

    In general, the selection of the immunogen varies from antibody to antibody. Some antibodies are raised against synthetic peptides (based ona protein sequence which was derived originally from a DNA sequence), some against protein fragments and others against the full length protein (either purified or overexpressed in another organism) or a cell preparation.

    Which specific antibody or protein target do you have in mind?

    As for the anti-IgA antibodies - the immunogendepends on the antibody.

    Please let me know more details about the products of your interest and I'd be happy to assist you further.

    Read More

    Answer

    I am sorry to hear that you have had problems with the 5 units of ab8519 that you purchased via VWR. the antibody has been tested in ELISA and Dot Blot only, not in an Ouchterlony gel. However I see no reason why the antibody should not work in this application and wish to help you resolve the problem. To this end, I would be grateful if you could answer the following questions: Have you purchased this product previously and found it to work successfully in this application? Have you tried all 5 vials of the antibody? How did the product reach you from VWR, was is shipped at 4 deg C? Please let me know the specification of the other rabbit antibody that you have found to work successfully in this application. I look forward to hearing from you.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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