Key features and details
- Rabbit polyclonal Secondary Antibody to Sheep IgG - Fc
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Product nameRabbit Anti-Sheep IgG Fc
See all IgG secondary antibodies
DescriptionRabbit polyclonal Secondary Antibody to Sheep IgG - Fc
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with sheep IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
Tested applicationsSuitable for: ICC, IHC-P, ELISA, WBmore details
Other Immunogen Type corresponding to IgG.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Our Abpromise guarantee covers the use of ab102297 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/200 - 1/2000.|
|IHC-P||1/200 - 1/2000.|
|ELISA||1/100 - 1/30000. (Coating: 1/100 - 1/500; Primary: 1/1000 - 1/30000).|
|WB||1/1000 - 1/30000. (Colorimetric: 1/1000 - 1/10000; Chemiluminescent: 1/1000 - 1/30000).|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab102297 has been referenced in 1 publication.
- Subharat S et al. Vaccination of Sheep with a Methanogen Protein Provides Insight into Levels of Antibody in Saliva Needed to Target Ruminal Methanogens. PLoS One 11:e0159861 (2016). ELISA ; Sheep . PubMed: 27472482