Rabbit Anti-Sheep IgG H&L (Alkaline Phosphatase) (ab6748)

Submit an Abreview Q&A (2)Specific References (2)

Overview

  • Product name
    Rabbit Anti-Sheep IgG H&L (Alkaline Phosphatase)
    See all IgG secondary antibodies
  • Host species
    Rabbit
  • Target species
    Sheep
  • Tested applications
    Suitable for: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Frmore details
  • Immunogen

    Sheep IgG whole molecule

  • Conjugation
    Alkaline Phosphatase

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer
    pH: 8
    Preservative: 0.1% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.014% Zinc chloride, 50% Glycerol
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads
  • Clonality
    Polyclonal
  • General notes

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons).

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
ELISA
Immunomicroscopy
Dot blot
ICC/IF
IHC-Fr
  • Application notes
    suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency

    • References

    This product has been referenced in:
    • Corti D  et al. Analysis of memory B cell responses and isolation of novel monoclonal antibodies with neutralizing breadth from HIV-1-infected individuals. PLoS One 5:e8805 (2010). ELISA ; Human . Read more (PubMed: 20098712) »
    • Richardson MR  et al. Diabetic dyslipidemia and exercise alter the plasma low-density lipoproteome in Yucatan pigs. Proteomics 9:2468-83 (2009). WB . Read more (PubMed: 19402046) »

    See all 2 Publications for this product

    Publishing research using ab6748? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    2 reviews or Q&A

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    Votes: Highest First

    I dont know if this should be posted via your webpage or not? Nevertheless, here is our answers to your questions: We have not tested ab6748 without any blocking agent and you are right this would also be a nice control to do. On the other hand we ...

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    Thank you for your enquiry. My colleague Dr Sarah Mardle is out of office until Thursday morning. As soon as she gets back, she will get in touch with you and deal with your enquiry. Thank you for your patience.

    BATCH NUMBER 67077 ORDER NUMBER 71216 DESCRIPTION OF THE PROBLEM We have recently used your antibody ab6748 (Rabbit polyclonal to Sheep IgG (alkaline phosphatase)) and have serious problems with it. Initially we used it as a secon...

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    I'm very sorry to hear you are having problems with ab6748. We take your complaint very seriously and would appreciate if you could please clarify whether you have tested the antibody with no blocking at all and tell me what permeabilising agent has be...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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