Key features and details
- Rabbit Anti-Sheep IgG H&L (Alkaline Phosphatase) preadsorbed
- Conjugation: Alkaline Phosphatase
- Host species: Rabbit
- Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Immunomicroscopy, Dot blot
Product nameRabbit Anti-Sheep IgG H&L (Alkaline Phosphatase) preadsorbed
See all IgG secondary antibodies
Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Immunomicroscopy, Dot blotmore details
HumanTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Full length native Sheep IgG (purified).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 8.00
Preservative: 0.01% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol, 0.87% Sodium chloride, 1% BSA
Concentration information loading...
Purification notesAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Conjugation notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads
Our Abpromise guarantee covers the use of ab7112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/200 - 1/1000.|
|IHC-P||Use at an assay dependent dilution.|
|IHC-Fr||Use at an assay dependent dilution.|
|WB||1/400 - 1/1600.|
|ELISA||1/1000 - 1/5000.|
|Immunomicroscopy||Use at an assay dependent dilution.|
|Dot blot||Use at an assay dependent dilution.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab7112 has not yet been referenced specifically in any publications.