Overview

  • Product name

    Rabbit Anti-Sheep IgG H&L (FITC)
    See all IgG secondary antibodies
  • Host species

    Rabbit
  • Target species

    Sheep
  • Tested applications

    Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
  • Immunogen

    Sheep whole molecule

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Clonality

    Polyclonal
  • General notes

    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.

References

This product has been referenced in:

  • Zhou QL  et al. FPR1 gene silencing suppresses cardiomyocyte apoptosis and ventricular remodeling in rats with ischemia/reperfusion injury through the inhibition of MAPK signaling pathway. Exp Cell Res 370:506-518 (2018). Read more (PubMed: 30031130) »
  • Mendoza JL  et al. The IFN-?-IFN-?R1-IL-10Rß Complex Reveals Structural Features Underlying Type III IFN Functional Plasticity. Immunity 46:379-392 (2017). Read more (PubMed: 28329704) »
See all 10 Publications for this product

Customer reviews and Q&As

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For IHC-P

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
I tried 1:100 and 1:500 dilutions for this antibody. 1:500 works well with an hour incubation at room temperature.

Abcam user community

Verified customer

Submitted Aug 20 2014

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