Overview

  • Product name

    Rabbit Anti-Sheep IgG H&L (Glucose Oxidase)
    See all IgG secondary antibodies
  • Description

    Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (Glucose Oxidase)
  • Host species

    Rabbit
  • Target species

    Sheep
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, ELISA, ICC, WBmore details
  • Immunogen

    Highly purified whole immunoglobulin from Sheep.

  • Conjugation

    Glucose Oxidase

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab136721 is purified by a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography).
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Do not store in plastic tubes (i.e. eppendorf) to avoid losing activity.

    Preparation of Substrate Solutions:

    ELISA: 10mg/ml D(+)glucose, 0.1mg/ml grade II (Rz=0.6) peroxidase, 0.35mg/ml OPD in 0.1M pH 5 citrate-phosphate buffer. Incubation 30 minutes at 37°C. Stop the reaction with V/2 volume of 2N H2SO4. Reading at 492 nm.

    Immunocytochemistry: 15mg/ml D(+)glucose, 0.5mg/ml blue thiazolyl, 0.35mg/ml phenazine methosulfonate to be dissolved in that order in a 0.1M pH 6.8 phosphate buffer. Incubation 20 to 30 minutes in darkness.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab136721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/100.
IHC-Fr 1/10 - 1/100.
ELISA 1/100 - 1/1000.
ICC 1/10 - 1/100.
WB 1/400.

References

ab136721 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab136721.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up