Overview

  • Product name

    Rabbit Anti-Sheep IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species

    Rabbit
  • Target species

    Sheep
  • Tested applications

    Suitable for: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Frmore details
  • Immunogen

    Sheep IgG whole molecule

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Horseradish Peroxidase (HRP).

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/1000 - 1/4000. PubMed: 15319485
ELISA Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ICC/IF 1/500 - 1/2500.
IHC-Fr Use at an assay dependent concentration.

Images

  • Ab6747 was used at dilution 1/2000 with the primary antibody ab7832 in WB. See the review on ab7832.
  • Representative immunohistochemical staining of parietal peritoneum tissue sections displaying HA in brown color (A-D). HA is dramatically reduced in the peritoneum from mice treated with PDF (B) compared to tissue from untreated mice (A) and mice pretreated with H398 2 hours before application of PDF (C) or PBS (D) (Scale bar: 100μm). The secondary antibody was Rabbit Anti-Sheep IgG H&L (HRP) (ab6747) at 1/100 dilution.

  • Ab6747 was used at dilution 1/100 with the primary antibody ab53842 in IHC-P. See the review on ab53842.

References

This product has been referenced in:

See all 24 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Gracias por contactarnos.

Lamentablemente no enviamos Certificados de Análisis de ninguno de nuestros productos.

Lo que si podemos enviaros, y los adjunto a este mail, son Certificados de Conformidad.

Espero que os sea de utilidad, y en cualquier caso no dudéis en contactarnos para cualquier duda o sugerencia.

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Answer

Thank you for your inquiry.

Please find attached the Certificates of Compliance for ab6747 and ab53586.

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