Overview

  • Product name

    Rabbit Anti-Sheep IgG H&L (HRP)
    See all IgG secondary antibodies
  • Description

    Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (HRP)
  • Host species

    Rabbit
  • Target species

    Sheep
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Sheep IgG and with light chains common to other Sheep immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applications

    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.05% Proclin
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notes

    Molar enzyme/ antibody protein ratio is 4:1
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/200 - 1/5000.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. (Primary)
WB 1/5000 - 1/50000. Colorimetric 1/5000 - 1/30000; Chemiluminescent 1/10000 - 1/50000

References

This product has been referenced in:

  • Pridans C  et al. Pleiotropic Impacts of Macrophage and Microglial Deficiency on Development in Rats with Targeted Mutation of the Csf1r Locus. J Immunol 201:2683-2699 (2018). Read more (PubMed: 30249809) »
  • Hjerpe R  et al. UBQLN2 Mediates Autophagy-Independent Protein Aggregate Clearance by the Proteasome. Cell 166:935-949 (2016). Read more (PubMed: 27477512) »
See all 5 Publications for this product

Customer reviews and Q&As

Answer

Thank you foryour email.

I am sorryfor theinconvenience this has caused. As requested, I have issued a free of charge replacement of one unit of ab97130 with the order number 1076961. The vial should arrive tomorrow at your place. To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

In order to complete our records, can you please confirm the following in regards to the ab7111:
The first vial was contaminated. The second vial had no contamination. Both vials have been tested at 1/1000 dilution and have not provided any signal.
Thankyou for confirming this. I am looking forward to your reply.

I wish you the best of luck with your research.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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