Overview

  • Product name
    Rabbit Anti-Sheep IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Rabbit
  • Target species
    Sheep
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Frmore details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    Full length native Sheep IgG (purified).

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    Horseradish Peroxidase (HRP)
  • Conjugation notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/300 - 1/1500.
IHC-P Use at an assay dependent dilution.
WB 1/500 - 1/3000.
ELISA 1/5000 - 1/22000.
Immunomicroscopy Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

References

This product has been referenced in:
  • Kristiansson MH  et al. Comparative time-dependent analysis of potential inflammation biomarkers in lymphoma-bearing SJL mice. J Proteome Res 6:1735-44 (2007). Read more (PubMed: 17388619) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for your answer.

We had checked the lot GR5200-6 and have not seen a contamination, this is why we have sent you one vial of this lot.

I am very sorry however to hear that the new vial does not work for you. We will be pleased to send you an alternative antibody as a replacement as we do not have any other lots of this antibody in stock. Can you please confirm in what dilution you have tested this antibody and it does not work?

As alternative antibodies, I can recommend the ab97130 or the ab6747 for example.https://www.abcam.com/index.html?datasheet=97130 (or use the following: https://www.abcam.com/index.html?datasheet=97130). https://www.abcam.com/index.html?datasheet=6747 (or use the following: https://www.abcam.com/index.html?datasheet=6747).

Please do let me know whether you would like to accept an alternative antibody or if you prefer to receive a credit note or refund.

I am looking forward to hear back from you.

Read More

Answer

Thank you foryour email.

I am sorryfor theinconvenience this has caused. As requested, I have issued a free of charge replacement of one unit of ab97130 with the order number 1076961. The vial should arrive tomorrow at your place. To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

In order to complete our records, can you please confirm the following in regards to the ab7111:
The first vial was contaminated. The second vial had no contamination. Both vials have been tested at 1/1000 dilution and have not provided any signal.
Thankyou for confirming this. I am looking forward to your reply.

I wish you the best of luck with your research.

Read More

Question
Answer

Thank you for having contacted us with the contamination problem with a vial of ab7111. I would like to apologize again for this error.

We have sent you a replacement vial, which should have arrived last Wednesday. I am writing you now, to see whetherit arrived well and to be reassured that you are satisfied with the new vial.

Thank you for a short feedback. I am looking forward to hear back from you.

I wish you all the best of success for your research.

Read More

Question
Answer

Thank you for your phone call. Unfortunately the vial we had ready to ship for you was also contaminated upon check. We are receiving however today the new stock from the our US office. I can unfortunately not guartantee that we can ship it out today still. I am very sorry. I will update you as soon as I know more.

The order number is xx and will be shipped as soon as possible. The new vial will be from batch xx and will be also in an amber vial. We changed the vials recently, just to be sure that long exposure to light would not damage the conjugate.

Please do not hesitate to contact me should you have any question. I thank you for your cooperation and understanding.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up