Rabbit Anti-Sheep IgG H&L (Texas Red ®) (ab6745)
Key features and details
- Rabbit Anti-Sheep IgG H&L (Texas Red ®)
- Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
- Host species: Rabbit
- Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA
Related conjugates and formulations
Overview
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Product name
Rabbit Anti-Sheep IgG H&L (Texas Red ®)
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Sheep -
Tested applications
Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISAmore details -
Immunogen
Sheep IgG whole molecule
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Conjugation
Texas Red ®. Ex: 596nm, Em: 620nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities -
Clonality
Polyclonal -
General notes
Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 4.9 moles Texas Redä per mole of Rabbit IgG
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Immunomicroscopy |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent dilution.
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| IHC-P |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
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| ICC/IF |
1/1000 - 1/5000. PubMed: 17895403
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| ELISA |
1/10000 - 1/50000.
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| Notes |
|---|
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Immunomicroscopy
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent dilution. |
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IHC-P
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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ICC/IF
1/1000 - 1/5000. PubMed: 17895403 |
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ELISA
1/10000 - 1/50000. |
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab6745 has been referenced in 6 publications.
- Yelland T et al. Stabilization of the RAS:PDE6D Complex Is a Novel Strategy to Inhibit RAS Signaling. J Med Chem 65:1898-1914 (2022). PubMed: 35104933
- Laber S et al. Linking the FTO obesity rs1421085 variant circuitry to cellular, metabolic, and organismal phenotypes in vivo. Sci Adv 7:N/A (2021). PubMed: 34290091
- Kirby BS et al. Robust internal elastic lamina fenestration in skeletal muscle arteries. PLoS One 8:e54849 (2013). IHC . PubMed: 23359815
- Pawlak E et al. Distribution and processing of a disintegrin and metalloproteinase with thrombospondin motifs-4, aggrecan, versican, and hyaluronan in equine digital laminae. Am J Vet Res 73:1035-46 (2012). PubMed: 22738056
- Jones S et al. Peripheral tachykinins and the neurokinin receptor NK1 are required for platelet thrombus formation. Blood 111:605-12 (2008). ICC/IF . PubMed: 17895403
- Czaja K et al. Leptin receptors, NPY, and tyrosine hydroxylase in autonomic neurons supplying fat depots in a pig. Biochem Biophys Res Commun 293:1138-44 (2002). PubMed: 12051778