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    rabbit-sheep-igg-hl-texas-red--ab6745.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Rabbit Anti-Sheep IgG H&L (Texas Red ®) (ab6745)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (6)

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Immunocytochemistry - Rabbit Anti-Sheep IgG H&L (Texas Red ®) (ab6745)

    Key features and details

    • Rabbit Anti-Sheep IgG H&L (Texas Red ®)
    • Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
    • Host species: Rabbit
    • Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Alkaline Phosphatase Biotin FITC Glucose Oxidase Gold 10nm Gold 15nm HRP TRITC Unconjugated Unconjugated

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    Overview

    • Product name

      Rabbit Anti-Sheep IgG H&L (Texas Red ®)
      See all IgG secondary antibodies
    • Host species

      Rabbit
    • Target species

      Sheep
    • Tested applications

      Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISAmore details
    • Immunogen

      Sheep IgG whole molecule

    • Conjugation

      Texas Red ®. Ex: 596nm, Em: 620nm

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
    • Concentration information loading...
    • Purity

      Affinity purified
    • Purification notes

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    • Conjugation notes

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
    • Clonality

      Polyclonal
    • General notes

      Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 4.9 moles Texas Redä per mole of Rabbit IgG

    • Research areas

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Secondary antibodies
      • anti-Sheep
      • IgG
      • Fluorophore
      • Texas Red

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6745 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Immunomicroscopy
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent dilution.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ICC/IF
    1/1000 - 1/5000. PubMed: 17895403
    ELISA
    1/10000 - 1/50000.
    Notes
    Immunomicroscopy
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent dilution.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ICC/IF
    1/1000 - 1/5000. PubMed: 17895403
    ELISA
    1/10000 - 1/50000.

    Images

    • Immunocytochemistry - Rabbit Anti-Sheep IgG H&L (Texas Red ®) (ab6745)
      Immunocytochemistry - Rabbit Anti-Sheep IgG H&L (Texas Red ®) (ab6745)
      Ab6745 was used at dilution 1/100 with the primary antibody ab30524 in ICC/IF. See the review on ab30524.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (6)

    Publishing research using ab6745? Please let us know so that we can cite the reference in this datasheet.

    ab6745 has been referenced in 6 publications.

    • Yelland T  et al. Stabilization of the RAS:PDE6D Complex Is a Novel Strategy to Inhibit RAS Signaling. J Med Chem 65:1898-1914 (2022). PubMed: 35104933
    • Laber S  et al. Linking the FTO obesity rs1421085 variant circuitry to cellular, metabolic, and organismal phenotypes in vivo. Sci Adv 7:N/A (2021). PubMed: 34290091
    • Kirby BS  et al. Robust internal elastic lamina fenestration in skeletal muscle arteries. PLoS One 8:e54849 (2013). IHC . PubMed: 23359815
    • Pawlak E  et al. Distribution and processing of a disintegrin and metalloproteinase with thrombospondin motifs-4, aggrecan, versican, and hyaluronan in equine digital laminae. Am J Vet Res 73:1035-46 (2012). PubMed: 22738056
    • Jones S  et al. Peripheral tachykinins and the neurokinin receptor NK1 are required for platelet thrombus formation. Blood 111:605-12 (2008). ICC/IF . PubMed: 17895403
    • Czaja K  et al. Leptin receptors, NPY, and tyrosine hydroxylase in autonomic neurons supplying fat depots in a pig. Biochem Biophys Res Commun 293:1138-44 (2002). PubMed: 12051778

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Hello I am working on a project to identify human cells in sheep tissues, specifically cardiac muscle, skeletal muscle, and liver. We are interested in your product ab259, the sheep polyclonal ab to human transferrin. What kind of cross reactivity does this antibody have to sheep? What are typical dilutions when using the ab for immunocytochemistry? What concentration does the ab come in? Finally, can you recommend another ab for identifying human cells in sheep? Thanks for your time! Evan Colletti

    Read More

    Abcam community

    Verified customer

    Asked on Mar 22 2002

    Answer

    This antibody has not been tested in sheep but is unlikely to cross-react as this polyclonal was raised in this species. The IgG concentration, however, should be in the region of 18.6mg/ml - as this is an IgG fraction, specific IgG will be approximately 10% of this.

    Read More

    Danielle Miller

    Abcam Scientific Support

    Answered on Mar 26 2002

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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