Rabbit Anti-Sheep IgG H&L (TRITC) (ab6744)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameRabbit Anti-Sheep IgG H&L (TRITC)
See all IgG secondary antibodies -
Host speciesRabbit
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Target speciesSheep
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Tested applicationsSuitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
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Immunogen
Sheep IgG whole molecule
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ConjugationTRITC. Ex: 547nm, Em: 572nm
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate -
Concentration information loading... -
PurityAffinity purified
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Purification notes.This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Conjugation notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
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ClonalityPolyclonal
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General notes
Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.8 moles TRITC per mole of Rabbit IgG.
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Research areas
Applications
Our Abpromise guarantee covers the use of ab6744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Immunomicroscopy | ||
| Flow Cyt | ||
| ICC/IF | ||
| IHC-P | ||
| IHC-Fr | ||
| ELISA |
Application notes
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency
References
This product has been referenced in:
- Gunawan RC et al. Complementary targeting of liposomes to IL-1a and TNF-a activated endothelial cells via the transient expression of VCAM1 and E-selectin. Biomaterials 32:9848-53 (2011). Read more (PubMed: 21944721) »
