Rabbit Anti-Sheep IgG H&L (TRITC) (ab6744)

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Key features and details

  • Rabbit Anti-Sheep IgG H&L (TRITC)
  • Conjugation: TRITC. Ex: 547nm, Em: 572nm
  • Host species: Rabbit
  • Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA

Overview

  • Product name

    Rabbit Anti-Sheep IgG H&L (TRITC)
    See all IgG secondary antibodies

  • Host species

    Rabbit

  • Target species

    Sheep

  • Tested applications

    Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details

  • Immunogen

    Sheep IgG whole molecule

  • Conjugation

    TRITC. Ex: 547nm, Em: 572nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    .This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • Conjugation notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities

  • Clonality

    Polyclonal

  • General notes

    Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.8 moles TRITC per mole of Rabbit IgG.

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy
Flow Cyt
ICC/IF
IHC-P
IHC-Fr
ELISA
Notes
Application notes
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab6744? Please let us know so that we can cite the reference in this datasheet.

ab6744 has been referenced in 1 publication.

  • Gunawan RC  et al. Complementary targeting of liposomes to IL-1a and TNF-a activated endothelial cells via the transient expression of VCAM1 and E-selectin. Biomaterials 32:9848-53 (2011). PubMed: 21944721

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