Rabbit Anti-Sheep IgG+IgM+IgA H&L (HRP) (ab8527)

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Overview

  • Product name
    Rabbit Anti-Sheep IgG+IgM+IgA H&L (HRP)
    See all IgG + IgM + IgA secondary antibodies
  • Host species
    Rabbit
  • Target species
    Sheep
  • Specificity
    Antibodies were raised in rabbit to Sheep IgG, IgM and IgA
  • Tested applications
    Suitable for: ELISA, Dot blotmore details
  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.50
    Constituents: 0.164% Sodium phosphate, 50% Glycerol, 0.58% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Specific antibodies were absorpted by incubation with Sepharose-bound Sheep immunoglobulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized Sheep immunoglobulins a minimum of 65% protein bound.
  • Conjugation notes
    The purified polyclonal was conjugated to horseradish peroxidase according to the periodate method followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
Dot blot 1/100.

References

ab8527 has not yet been referenced specifically in any publications.

Publishing research using ab8527? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

Has ab 8527 ever been used as a primary antibody in IHC.

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Answer

To our knowledge, this antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

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Question

Can this product be used for western blotting, and if so what dilution is recommended.

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Answer

This antibody has never been tested in WB, therefore we can't guarantee results.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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