Product nameRabbit specific HRP/AEC IHC Detection Kit - Micro-polymer
See all HRP/AEC Detection Kit kits
This IHC detection kit is used to detect rabbit primary antibodies in immunohistochemistry (IHC). It uses an HRP micro-polymer secondary antibody for highly sensitive detection.
HRP micro-polymer methods are more sensitive than the traditional streptavidin-biotin method, and they do not require a blocking step for endogenous biotin.
It is an exact copy of EXPOSE Rabbit specific HRP-AEC detection IHC Kit ab94361, except that it uses the HRP micro-polymer antibody used during the validation of Abcam's antibodies in IHC. This HRP micro-polymer antibody was identified by our R&D team as giving the most sensitive and specific staining.
The kit includes ready-to-use hydrogen peroxide blocking reagent, protein blocking reagent, HRP micro-polymer conjugated goat anti-rabbit secondary antibody, and AEC chromogen.
Tested applicationsSuitable for: IHC-Pmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 15 ml AEC Single Solution 1 x 15ml Goat anti-rabbit HRP Conjugate 1 x 15ml Hydrogen Peroxide Block 1 x 15ml Protein block 1 x 15ml
RelevanceAn HRP/AEC Detection Kit is a labeled immunoenzymatic antigen detection system. Horseradish peroxidase (HRP) readily combines with hydrogen peroxide (H2O2) and the resultant complex can oxidize a wide variety of chromogenic substrates. AEC is 3-amino-9-ethyl-carbazole, a chromogenic substrate.
- Horseradish Peroxidase
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab236468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
ab236468 has not yet been referenced specifically in any publications.