Overview

  • Product name

    Rabbit specific HRP/AEC IHC Detection Kit - Micro-polymer
    See all HRP/AEC Detection Kit kits
  • Detection method

    Colorimetric
  • Assay type

    Micro-Polymer
  • Product overview

    This IHC detection kit is used to detect rabbit primary antibodies in immunohistochemistry (IHC). It uses an HRP micro-polymer secondary antibody for highly sensitive detection.


    HRP micro-polymer methods are more sensitive than the traditional streptavidin-biotin method, and they do not require a blocking step for endogenous biotin.


    It is an exact copy of EXPOSE Rabbit specific HRP-AEC detection IHC Kit ab94361, except that it uses the HRP micro-polymer antibody used during the validation of Abcam's antibodies in IHC. This HRP micro-polymer antibody was identified by our R&D team as giving the most sensitive and specific staining.


    The kit includes ready-to-use hydrogen peroxide blocking reagent, protein blocking reagent, HRP micro-polymer conjugated goat anti-rabbit secondary antibody, and AEC chromogen.

  • Notes

    For alternative kits, see Anti-Mouse / anti-Rabbit HRP-AEC Micro-polymer IHC Kit ab236467, and HRP-DAB micro-polymer IHC kits.

    Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • Tested applications

    Suitable for: IHC-Pmore details

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 15 ml
    AEC Single Solution 1 x 15ml
    Goat anti-rabbit HRP Conjugate 1 x 15ml
    Hydrogen Peroxide Block 1 x 15ml
    Protein block 1 x 15ml
  • Research areas

  • Relevance

    An HRP/AEC Detection Kit is a labeled immunoenzymatic antigen detection system. Horseradish peroxidase (HRP) readily combines with hydrogen peroxide (H2O2) and the resultant complex can oxidize a wide variety of chromogenic substrates. AEC is 3-amino-9-ethyl-carbazole, a chromogenic substrate.
  • Alternative names

    • carbazole
    • Horseradish Peroxidase

Associated products

Applications

Our Abpromise guarantee covers the use of ab236468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Images

  • Immunohistochemical analysis of Human Tonsil tissue labeling Ki-67 using the ab94361 anti-EXPOSE Rabbit specific HRP-AEC detection IHC kit (this kit is identical to ab94361, except that it uses the HRP micro-polymer secondary antibody used by Abcam's R&D team for antibody validation in IHC).

Protocols

References

ab236468 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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