For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    rabbit-specific-hrpdab-detection-ihc-detection-kit-micro-polymer-ab236469.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Kits/ Lysates/ Other Kits IHC Kits
Share by email

Rabbit specific HRP/DAB Detection IHC Detection Kit - Micro-polymer (ab236469)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (2) Submit a question References (9)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific HRP/DAB Detection IHC Detection Kit - Micro-polymer (ab236469)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific HRP/DAB Detection IHC Detection Kit - Micro-polymer (ab236469)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

You may also be interested in

Western blot
Product image
InstantBlue® Coomassie Protein Stain (ISB1L) (ab119211)
Primary
Product image
Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191)
Primary
Product image
Anti-DFNA5/GSDME antibody [EPR19859] - BSA and Azide free (ab223877)

View more associated products

Overview

  • Product name

    Rabbit specific HRP/DAB Detection IHC Detection Kit - Micro-polymer
    See all HRP/DAB detection kit kits
  • Detection method

    Colorimetric
  • Assay type

    Micro-Polymer
  • Product overview

    This IHC kit is used to detect rabbit primary antibodies in immunohistochemistry (IHC). It uses an HRP micro-polymer secondary antibody for highly sensitive detection.


    HRP micro-polymer methods are more sensitive than the traditional streptavidin-biotin method, and they do not require a blocking step for endogenous biotin.


    This kit is an exact copy of EXPOSE Rabbit Specific HRP/DAB Detection IHC Kit ab80437, except that it uses the HRP micro-polymer antibody used during the validation of Abcam's antibodies in IHC. This HRP micro-polymer antibody was identified by our R&D team as giving the most sensitive and specific staining. 


    The kit includes ready-to-use hydrogen peroxide blocking reagent, protein blocking reagent, HRP micro-polymer conjugated goat anti-rabbit secondary antibody, and DAB chromogen/substrate.

  • Notes

    The previous version of this kit, the EXPOSE Rabbit Specific HRP/DAB Detection IHC Kit, was used by researchers to stain over 75,000 slides since 2012 (based on volumes of reagent sold).

     

    Other IHC detection products

    For alternative kits, see anti-Mouse / anti-Rabbit HRP-DAB Micro-polymer IHC Kit ab236466, and HRP-AEC micro-polymer IHC kits.

    Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • Tested applications

    Suitable for: IHC-Pmore details

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 15 ml 60 ml
    50x DAB Chromogen 1 x 0.5ml 1 x 2ml
    DAB substrate 1 x 15ml 1 x 60ml
    Goat anti-rabbit HRP Conjugate 1 x 15ml 1 x 60ml
    Hydrogen Peroxide Block 1 x 15ml 1 x 60ml
    Protein block 1 x 15ml 1 x 60ml
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • IHC Kits
  • Relevance

    HRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab236469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific HRP/DAB Detection IHC Detection Kit - Micro-polymer (ab236469)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific HRP/DAB Detection IHC Detection Kit - Micro-polymer (ab236469)Image from Uehara et al., Sci Rep., 10(1):4441; doi: 10.1038/s41598-020-61456-7. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
    ATM inhibition upregulated proinflammatory and profibrotic signaling in cisplatin nephropathy. a) Immunostaining of kidney sections for Kim-1 and megalin (4 days after treatment).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific HRP/DAB Detection IHC Detection Kit - Micro-polymer (ab236469)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific HRP/DAB Detection IHC Detection Kit - Micro-polymer (ab236469)
    ab236469 Rabbit Specific HRP-DAB IHC Detection Kit - Micro-Polymer on Human Tonsil staining CD3
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    ab209064 at 1:2000 staining TMEM119 antibody in mouse cerebrum tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TMEM119 with ab209064 at 1/2000 dilution followed by the same HRP micro-polymer goat anti-rabbit secondary antibody used in this kit. Positive staining on glial cells in mouse cerebrum is observed. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0) before commencing with IHC staining protocol. Counter stained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)Hall OJ et al. PLoS Pathog. 12:e1005840 (2016) Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunohistochemical analysis of mouse lung tissue labeling Ki67 detected with ab80437 (this kit is identical to ab80437, except that it uses the HRP micro-polymer secondary antibody used by Abcam's R&D team for antibody validation in IHC). Tissue sections were embedded in paraffin and were counterstained with hematoxylin. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)Selby MJ et al. PLoS One (2016)

    Immunohistochemical (frozen) analysis of MC38 tumor sections labeling CD3 with ab16669 at 1/400 dilution. Sections were fixed with acetone, treated with peroxidase block to quench endogenous peroxidase, and then further blocked with a 10% goat serum and 5% BSA solution. CD3 positive T cells were detected using ab80437 (this kit is identical to ab80437, except that it uses the HRP micro-polymer antibody used by Abcam's R&D team for antibody validation in IHC). Invasive margin (top) shows sections derived from the periphery of the tumor and interior (bottom) shows sections from within the tumor.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (9)

Publishing research using ab236469? Please let us know so that we can cite the reference in this datasheet.

ab236469 has been referenced in 9 publications.

  • Ahn KH  et al. Hyaluronic Acid Treatment Improves Healing of the Tenorrhaphy Site by Suppressing Adhesions through Extracellular Matrix Remodeling in a Rat Model. Polymers (Basel) 13:N/A (2021). PubMed: 33802991
  • Pandya AD  et al. Biodistribution of Poly(alkyl cyanoacrylate) Nanoparticles in Mice and Effect on Tumor Infiltration of Macrophages into a Patient-Derived Breast Cancer Xenograft. Nanomaterials (Basel) 11:N/A (2021). PubMed: 33924869
  • Kwon HK  et al. Dual therapeutic targeting of intra-articular inflammation and intracellular bacteria enhances chondroprotection in septic arthritis. Sci Adv 7:N/A (2021). PubMed: 34172438
  • Chow SKH  et al. Acute Inflammatory Response in Osteoporotic Fracture Healing Augmented with Mechanical Stimulation is Regulated In Vivo through the p38-MAPK Pathway. Int J Mol Sci 22:N/A (2021). PubMed: 34445423
  • Uehara M  et al. Pharmacological inhibition of ataxia-telangiectasia mutated exacerbates acute kidney injury by activating p53 signaling in mice. Sci Rep 10:4441 (2020). PubMed: 32157166
  • Kim YS  et al. Novel Medicine for Endometriosis and Its Therapeutic Effect in a Mouse Model. Biomedicines 8:N/A (2020). PubMed: 33339236
  • Recinella L  et al. Antinflammatory, antioxidant, and behavioral effects induced by administration of growth hormone-releasing hormone analogs in mice. Sci Rep 10:732 (2020). PubMed: 31959947
  • Torretta S  et al. D-mannose suppresses macrophage IL-1ß production. Nat Commun 11:6343 (2020). PubMed: 33311467
  • Gao G  et al. Effects of Hesperidin on H2O2-Treated Chondrocytes and Cartilage in a Rat Osteoarthritis Model. Med Sci Monit 24:9177-9186 (2018). PubMed: 30557884

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Rabbit specific HRP/DAB Detection IHC Detection Kit - Micro-polymer

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Staining was performed on paraffin embedded rat tissues. The kit is excellent. Providing low background staining. The only disadvantage is that mixing the amounts of DAB chromagen and substrate according to the attached protocol (1 drop and 50 drops, respectively), makes to much material that goes to waste when few tissues are stained at the same time.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Hani Nevo

Verified customer

Submitted May 13 2020

Rabbit specific HRP/DAB Detection IHC Detection Kit - Micro-polymer

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Staining was performed on paraffin embedded rat tissues. The kit is excellent. Providing low background staining. The only disadvantage is that mixing the amounts of DAB chromagen and substrate according to the attached protocol (1 drop and 50 drops, respectively), makes to much material that goes to waste when few tissues are stained at the same time.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Hani Nevo

Verified customer

Submitted May 12 2020

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.