Rabbit specific HRP/DAB Detection IHC Detection Kit - Micro-polymer (ab236469)

Overview

  • Product name
    Rabbit specific HRP/DAB Detection IHC Detection Kit - Micro-polymer
    See all HRP/DAB detection kit kits
  • Detection method
    Colorimetric
  • Assay type
    Micro-Polymer
  • Product overview

    This IHC kit is used to detect rabbit primary antibodies in immunohistochemistry (IHC). It uses an HRP micro-polymer secondary antibody for highly sensitive detection.


    HRP micro-polymer methods are more sensitive than the traditional streptavidin-biotin method, and they do not require a blocking step for endogenous biotin.


    This kit is an exact copy of EXPOSE Rabbit Specific HRP/DAB Detection IHC Kit ab80437, except that it uses the HRP micro-polymer antibody used during the validation of Abcam's antibodies in IHC. This HRP micro-polymer antibody was identified by our R&D team as giving the most sensitive and specific staining. 


    The kit includes ready-to-use hydrogen peroxide blocking reagent, protein blocking reagent, HRP micro-polymer conjugated goat anti-rabbit secondary antibody, and DAB chromogen/substrate.

  • Notes

    The previous version of this kit, the EXPOSE Rabbit Specific HRP/DAB Detection IHC Kit, was used by researchers to stain over 75,000 slides since 2012 (based on volumes of reagent sold).

     

    Other IHC detection products

    For alternative kits, see anti-Mouse / anti-Rabbit HRP-DAB Micro-polymer IHC Kit ab236466, and HRP-AEC micro-polymer IHC kits.

    Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • Tested applications
    Suitable for: IHC-Pmore details

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 15 ml 60 ml
    50x DAB Chromogen 1 x 0.5ml 1 x 2ml
    DAB substrate 1 x 15ml 1 x 60ml
    Goat anti-rabbit HRP Conjugate 1 x 15ml 1 x 60ml
    Hydrogen Peroxide Block 1 x 15ml 1 x 60ml
    Protein block 1 x 15ml 1 x 60ml
  • Research areas
  • Relevance
    HRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.

Applications

Our Abpromise guarantee covers the use of ab236469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Images

  • ab236469 Rabbit Specific HRP-DAB IHC Detection Kit - Micro-Polymer on Human Tonsil staining CD3
  • ab209064 at 1:2000 staining TMEM119 antibody in mouse cerebrum tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TMEM119 with ab209064 at 1/2000 dilution followed by the same HRP micro-polymer goat anti-rabbit secondary antibody used in this kit. Positive staining on glial cells in mouse cerebrum is observed. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0) before commencing with IHC staining protocol. Counter stained with hematoxylin.

  • Immunohistochemical analysis of mouse lung tissue labeling Ki67 detected with ab80437 (this kit is identical to ab80437, except that it uses the HRP micro-polymer secondary antibody used by Abcam's R&D team for antibody validation in IHC). Tissue sections were embedded in paraffin and were counterstained with hematoxylin. 

  • Immunohistochemical (frozen) analysis of MC38 tumor sections labeling CD3 with ab16669 at 1/400 dilution. Sections were fixed with acetone, treated with peroxidase block to quench endogenous peroxidase, and then further blocked with a 10% goat serum and 5% BSA solution. CD3 positive T cells were detected using ab80437 (this kit is identical to ab80437, except that it uses the HRP micro-polymer antibody used by Abcam's R&D team for antibody validation in IHC). Invasive margin (top) shows sections derived from the periphery of the tumor and interior (bottom) shows sections from within the tumor.

Protocols

References

This product has been referenced in:
  • Gao G  et al. Effects of Hesperidin on H2O2-Treated Chondrocytes and Cartilage in a Rat Osteoarthritis Model. Med Sci Monit 24:9177-9186 (2018). Read more (PubMed: 30557884) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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