Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Overview
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Product name
Rabbit specific IHC polymer detection kit HRP/DAB -
Product overview
Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) is a sensitive immunoenzymatic system for detecting antibodies bound to antigens within tissue sections by light microscopy. Signal amplification is achieved using an indirect polymer detection system. As Rabbit specific IHC polymer detection kit HRP/DAB does not use avidin or biotin, nonspecific staining from endogenous avidin-biotin activity is eliminated and there is no need for additional steps to block endogenous biotin. The primary antibody specific to an antigen on the tissue section is detected by primary antibody Amplifier. The HRP polymer is added to enable detection with signal amplification. Finally, antibody-bound antigen sites are visualized by adding an appropriate substrate/chromogen (not provided).
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Tested applications
Suitable for: IHCmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 15 ml Amplifier 1 x 15ml Detector 1 x 15ml
Associated products
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab209101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC
Use at an assay dependent concentration. |
Images
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Immunohistochemistry paraffin embedded sections - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling USP22 with ab195289 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat cerebrum. The section was incubated with ab195289 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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Immunohistochemistry paraffin embedded sections - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling USP22 with ab195289 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse cerebrum. The section was incubated with ab195289 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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Immunohistochemistry paraffin embedded sections - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling USP22 with ab195289 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human breast carcinoma. The section was incubated with ab195289 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemistry paraffin embedded sections - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling USP22 with ab195289 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human cerebrum. The section was incubated with ab195289 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemistry paraffin embedded sections - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling HSF1 with ab52757 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse colon. The section was incubated with ab52757 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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Immunohistochemistry paraffin embedded sections - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling HSF1 with ab52757 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse testis. The section was incubated with ab52757 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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ab209101 MelanomaImmunohistochemistry of human melanoma tissue (formalin/PFA-fixed paraffin embedded sections) stained with Anti-PD-L1 antibody [28-8] (ab205921) and detected with Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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ab209101 TonsilImmunohistochemistry of human tonsil tissue (formalin/PFA-fixed paraffin embedded sections) stained with Anti-PD-L1 antibody [28-8] (ab205921) and detected with Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab209101 has been referenced in 10 publications.
- Mei J et al. A comparability study of natural and deglycosylated PD-L1 levels in lung cancer: evidence from immunohistochemical analysis. Mol Cancer 20:11 (2021). PubMed: 33413365
- Ozawa N et al. PD-L1 upregulation is associated with activation of the DNA double-strand break repair pathway in patients with colitic cancer. Sci Rep 11:13077 (2021). IHC-P ; Human . PubMed: 34158547
- Zhu H et al. Asah2 Represses the p53-Hmox1 Axis to Protect Myeloid-Derived Suppressor Cells from Ferroptosis. J Immunol 206:1395-1404 (2021). PubMed: 33547170
- Younis RH et al. Soluble Sema4D in Plasma of Head and Neck Squamous Cell Carcinoma Patients Is Associated With Underlying Non-Inflamed Tumor Profile. Front Immunol 12:596646 (2021). PubMed: 33776991
- Guo G et al. miR-7/SP1/TP53BP1 axis may play a pivotal role in NSCLC radiosensitivity. Oncol Rep 44:2678-2690 (2020). IHC-P ; Human . PubMed: 33125142
- Wang S et al. Raw and salt-processed Achyranthes bidentata attenuate LPS-induced acute kidney injury by inhibiting ROS and apoptosis via an estrogen-like pathway. Biomed Pharmacother 129:110403 (2020). PubMed: 32574970
- Lu C et al. SUV39H1 Represses the Expression of Cytotoxic T-Lymphocyte Effector Genes to Promote Colon Tumor Immune Evasion. Cancer Immunol Res 7:414-427 (2019). PubMed: 30610059
- Aotsuka A et al. Interleukin-17 is associated with expression of programmed cell death 1 ligand 1 in ovarian carcinoma. Cancer Sci 110:3068-3078 (2019). PubMed: 31432577
- Zhao S et al. TLR4 expression correlated with PD-L1 expression indicates a poor prognosis in patients with peripheral T-cell lymphomas. Cancer Manag Res 11:4743-4756 (2019). PubMed: 31191027
- Sato Y et al. The PD-1/PD-L1 axis may be aberrantly activated in occupational cholangiocarcinoma. Pathol Int N/A:N/A (2017). PubMed: 28139862