Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Rabbit specific IHC polymer detection kit HRP/DAB -
Product overview
Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) is a sensitive immunoenzymatic system for detecting antibodies bound to antigens within tissue sections by light microscopy. Signal amplification is achieved using an indirect polymer detection system. As Rabbit specific IHC polymer detection kit HRP/DAB does not use avidin or biotin, nonspecific staining from endogenous avidin-biotin activity is eliminated and there is no need for additional steps to block endogenous biotin. The primary antibody specific to an antigen on the tissue section is detected by primary antibody Amplifier. The HRP polymer is added to enable detection with signal amplification. Finally, antibody-bound antigen sites are visualized by adding an appropriate substrate/chromogen (not provided).
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Tested applications
Suitable for: IHCmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 15 ml Amplifier 1 x 15ml Detector 1 x 15ml
Associated products
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Related Products
Applications
Our Abpromise guarantee covers the use of ab209101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC | Use at an assay dependent concentration. |
Images
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ab209101 MelanomaImmunohistochemistry of human melanoma tissue (formalin/PFA-fixed paraffin embedded sections) stained with Anti-PD-L1 antibody [28-8] (ab205921) and detected with Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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ab209101 TonsilImmunohistochemistry of human tonsil tissue (formalin/PFA-fixed paraffin embedded sections) stained with Anti-PD-L1 antibody [28-8] (ab205921) and detected with Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Protocols
References
This product has been referenced in:
- Zhao S et al. TLR4 expression correlated with PD-L1 expression indicates a poor prognosis in patients with peripheral T-cell lymphomas. Cancer Manag Res 11:4743-4756 (2019). Read more (PubMed: 31191027) »
- Sato Y et al. The PD-1/PD-L1 axis may be aberrantly activated in occupational cholangiocarcinoma. Pathol Int N/A:N/A (2017). Read more (PubMed: 28139862) »
