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Immunology Immunoglobulins Heavy Chain IgG
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Rabbit Anti-Syrian Hamster IgG H&L (Biotin) (ab6782)

  • Datasheet
  • SDS
Submit a review Q&A (3)References (6)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Syrian Hamster IgG H&L (Biotin) (ab6782)

    Key features and details

    • Rabbit Anti-Syrian Hamster IgG H&L (Biotin)
    • Conjugation: Biotin
    • Host species: Rabbit
    • Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WB

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    Overview

    • Product name

      Rabbit Anti-Syrian Hamster IgG H&L (Biotin)
      See all IgG secondary antibodies
    • Host species

      Rabbit
    • Target species

      Syrian hamster
    • Tested applications

      Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details
    • Immunogen

      hamster IgG whole molecule

    • Conjugation

      Biotin

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
    • Concentration information loading...
    • Purity

      Affinity purified
    • Purification notes

      Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule.
    • Conjugation notes

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
    • Clonality

      Polyclonal
    • Research areas

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Secondary antibodies
      • anti-Hamster
      • IgG
      • Biotin

    Associated products

      Applications

      The Abpromise guarantee

      Our Abpromise guarantee covers the use of ab6782 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Abreviews Notes
      Dot blot
      ELISA
      IHC-P
      IHC-Fr
      Immunomicroscopy
      ICC/IF
      WB
      Notes
    • Application notes
      immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency
    • Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Syrian Hamster IgG H&L (Biotin) (ab6782)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Syrian Hamster IgG H&L (Biotin) (ab6782)
        Ab6782 was used at dilution 1/800 with the primary antibody ab11936 in IHC-P. See the review on ab11936.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (6)

      Publishing research using ab6782? Please let us know so that we can cite the reference in this datasheet.

      ab6782 has been referenced in 6 publications.

      • Zhang M  et al. LncRNA SNHG5 affects cell proliferation, metastasis and migration of colorectal cancer through regulating miR-132-3p/CREB5. Cancer Biol Ther 20:524-536 (2019). PubMed: 30395767
      • Lee D  et al. HMGB2 is a novel adipogenic factor that regulates ectopic fat infiltration in skeletal muscles. Sci Rep 8:9601 (2018). PubMed: 29942000
      • Liu J  et al. Downregulation of let-7b promotes COL1A1 and COL1A2 expression in dermis and skin fibroblasts during heat wound repair. Mol Med Rep 13:2683-8 (2016). PubMed: 26861712
      • Alymova IV  et al. A novel cytotoxic sequence contributes to influenza A viral protein PB1-F2 pathogenicity and predisposition to secondary bacterial infection. J Virol 88:503-15 (2014). PubMed: 24173220
      • Guo Q  et al. Mouse lymphatic endothelial cell targeted probes: anti-LYVE-1 antibody-based magnetic nanoparticles. Int J Nanomedicine 8:2273-84 (2013). IHC-P . PubMed: 23818783
      • Barnes NL  et al. Cyclooxygenase-2 inhibition: effects on tumour growth, cell cycling and lymphangiogenesis in a xenograft model of breast cancer. Br J Cancer 96:575-82 (2007). PubMed: 17285134

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-3 of 3 Abreviews or Q&A

      Question

      Following our standard protocol for IHC, I could not detect specific staining for CD11c in spleen and ear (dermis) sections. A staining with anti MHC II that I performed in parallel worked just fine. Could you please suggest me how to improve my protocol? According to a paper stated in your bibliography, Metlay 1990 J. Exp. Med. 171:1753, I used acetone for fixation. 1. Take (cryo-)sections out the freezer and warm at RT for 30` 2. Fixation: in cuvette with ice-cold acetone 10` 3. Permeabilization and washing: 3 x 5` in PBS at RT, 1st wash with 0.2%TX-100 4. Blocking: 30` with 5% goat serum in PBS (500µl/slide) 5. 1st antibody: in 5% goat serum O/N, 4ºC (200µl/slide + parafilm) I used up to 1:50 dilution for the hamster anti-CD11c (20ng/ul), I controled the staining with sections, that did not receive 1st antibody. Also 1: 10,000 DAPI 6. Washing: 3 x 10` with PBS 7. 2nd antibody: in 2% goat serum O/N, 4ºC (200µl/slide + parafilm), I used your BIOTIN-anti Hamster 1:200 (5ng/ul) 8. Washing: 3 x 10` with PBS 9. ST-TRITC: in 2% goat serum O/N, 4ºC (200µl/slide + parafilm), I used a standard concentration of 1:200, that works well in other stainings 10. Washing: 2 x 10` in PBS 1 x 10` in Tris-HCl, pH 7.4 (remove salt) 11. Mounting: dry slides for a few minutes, Mowiol, store in dark at 4ºC I would be happpy to receive any suggestions,

      Read More

      Abcam community

      Verified customer

      Asked on Sep 01 2006

      Answer

      I apologise for the delay in responding to you, I have at last received feedback from the laboratory that tested ab33483 and include their response below: I have had a look through your customers protocol and note that it differs somewhat from our recommended protocol for cryostat sections. So firstly from their protocols: 1. I note sections appear to be stored in a freezer prior to staining. Some antigens are more sensitive to this than others, this may be the case here. I would recommend using freshly sectioned material 2. Following acetone fixation they have included a triton step (for the DAPI?). This may not be compatible with staining for CD11c using this antibody. I would suggest omitting this step. I note from the literature that such a step is not included in publications reporting successful staining with this antibody. 3. We do not support the use of Immunofluorescence for this antibody. I do not know whether there is sufficient sensitivity to allow the use of TRIT-C for its detection. I would suggest the use of a peroxidase-DAB protocol as has been used in all the publication reporting successful. My gut feeling is that it may be the Triton X-100 that may affect the epitope recognised by the antibody. Just omitting this step may help restore antigenicity. Please find a list of publications where this has been used successfully for IHC on cryostat sections. There may be further appropriate advice in these. 1: Witmer-Pack MD, Crowley MT, Inaba K, Steinman RM. Macrophages, but not dendritic cells, accumulate colloidal carbon following administration in situ. J Cell Sci. 1993 Aug;105 ( Pt 4):965-73. PMID: 7693737 2: Metlay JP, Witmer-Pack MD, Agger R, Crowley MT, Lawless D, Steinman RM. The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies. J Exp Med. 1990 May 1;171(5):1753-71. PMID: 2185332 3: McMenamin PG. Dendritic cells and macrophages in the uveal tract of the normal mouse eye. Br J Ophthalmol. 1999 May;83(5):598-604. PMID: 10216062 4: Pimorady-Esfahani A, Grounds MD, McMenamin PG. Macrophages and dendritic cells in normal and regenerating murine skeletal muscle. Muscle Nerve. 1997 Feb;20(2):158-66. PMID: 9040653 5: Kelsall BL, Strober W. Distinct populations of dendritic cells are present in the subepithelial dome and T cell regions of the murine Peyer's patch. J Exp Med. 1996 Jan 1;183(1):237-47. PMID: 8551227 6: Dahlen E, Dawe K, Ohlsson L, Hedlund G. Dendritic cells and macrophages are the first and major producers of TNF-alpha in pancreatic islets in the nonobese diabetic mouse. J Immunol. 1998 Apr 1;160(7):3585-93. PMID: 9531322 7: Leenen PJ, Radosevic K, Voerman JS, Salomon B, van Rooijen N, Klatzmann D, van Ewijk W. Heterogeneity of mouse spleen dendritic cells: in vivo phagocytic activity, expression of macrophage markers, and subpopulation turnover. J Immunol. 1998 Mar 1;160(5):2166-73. PMID: 9498754 8: Gonzalez-Juarrero M, Orme IM. Characterization of murine lung dendritic cells infected with Mycobacterium tuberculosis. Infect Immun. 2001 Feb;69(2):1127-33. PMID: 11160010 9: Hamada H, Hiroi T, Nishiyama Y, Takahashi H, Masunaga Y, Hachimura S, Kaminogawa S, Takahashi-Iwanaga H, Iwanaga T, Kiyono H, Yamamoto H, Ishikawa H. Identification of multiple isolated lymphoid follicles on the antimesenteric wall of the mouse small intestine. J Immunol. 2002 Jan 1;168(1):57-64. PMID: 11751946 10: Bjorck P. Dendritic cells exposed to herpes simplex virus in vivo do not produce IFN-alpha after rechallenge with virus in vitro and exhibit decreased T cell alloreactivity. J Immunol. 2004 May 1;172(9):5396-404. PMID: 15100280 Please note that most of these appear to be available freely via PubMed. Reference 1 has some great pictures of staining in the liver and in spleen with N418 and other antibodies such as F4/80 and FA-11 etc. I hope this advice will be useful. Please do not hesitate to contact me if you still experience problems with those recommendations, if you purchased the antibody in the last 90 days I would be happy to offer you a refund,

      Read More

      Abcam Scientific Support

      Answered on Sep 18 2006

      Question

      I found this information on the web: http://www.histosearch.com/histonet/Oct04/Re.HistonetBackgroundwithC.html where it is recommended to pre-absorb the secondary detecting a hamster monoclonal to CD11c. I bought both antibodies from you, please advise on how to use the secondary. Thanks

      Read More

      Abcam community

      Verified customer

      Asked on Aug 30 2006

      Answer

      Thank you for your enquiry. The protocol quoted in the link you kindly provided does not seem to mention our antibodies but antibodies from other companies. In our experience the secondary ab6782 does not give background problems and does not need to be pre-absorbed. If you experience any problems or need further advice regarding your IHC protocol please do not hesitate to contact me, I would be happy to look at your full protocol,

      Read More

      Abcam Scientific Support

      Answered on Aug 31 2006

      Question

      Is this Ab appropriate as a labeling secondary Ab (for IHC) to an Armenian hamster IgG, group 3, lamda (anti-mouse CD51 monoclonal antibody)

      Read More

      Abcam community

      Verified customer

      Asked on Jul 09 2001

      Answer

      This antibody is not recommended for Armenian hamster IgG.

      Read More

      Neil Hayward

      Abcam Scientific Support

      Answered on Jul 10 2001

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