Overview

  • Product name
    Rabbit Anti-Syrian Hamster IgG H&L (Biotin)
    See all IgG secondary antibodies
  • Host species
    Rabbit
  • Target species
    Syrian hamster
  • Tested applications
    Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details
  • Immunogen

    hamster IgG whole molecule

  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule.
  • Conjugation notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
ICC/IF
WB
  • Application notes
    immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency
  • Images

    • Ab6782 was used at dilution 1/800 with the primary antibody ab11936 in IHC-P. See the review on ab11936.

    References

    This product has been referenced in:
    • Lee D  et al. HMGB2 is a novel adipogenic factor that regulates ectopic fat infiltration in skeletal muscles. Sci Rep 8:9601 (2018). Read more (PubMed: 29942000) »
    • Liu J  et al. Downregulation of let-7b promotes COL1A1 and COL1A2 expression in dermis and skin fibroblasts during heat wound repair. Mol Med Rep 13:2683-8 (2016). Read more (PubMed: 26861712) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    I apologise for the delay in responding to you, I have at last received feedback from the laboratory that tested ab33483 and include their response below: I have had a look through your customers protocol and note that it differs somewhat from our recommended protocol for cryostat sections. So firstly from their protocols: 1. I note sections appear to be stored in a freezer prior to staining. Some antigens are more sensitive to this than others, this may be the case here. I would recommend using freshly sectioned material 2. Following acetone fixation they have included a triton step (for the DAPI?). This may not be compatible with staining for CD11c using this antibody. I would suggest omitting this step. I note from the literature that such a step is not included in publications reporting successful staining with this antibody. 3. We do not support the use of Immunofluorescence for this antibody. I do not know whether there is sufficient sensitivity to allow the use of TRIT-C for its detection. I would suggest the use of a peroxidase-DAB protocol as has been used in all the publication reporting successful. My gut feeling is that it may be the Triton X-100 that may affect the epitope recognised by the antibody. Just omitting this step may help restore antigenicity. Please find a list of publications where this has been used successfully for IHC on cryostat sections. There may be further appropriate advice in these. 1: Witmer-Pack MD, Crowley MT, Inaba K, Steinman RM. Macrophages, but not dendritic cells, accumulate colloidal carbon following administration in situ. J Cell Sci. 1993 Aug;105 ( Pt 4):965-73. PMID: 7693737 2: Metlay JP, Witmer-Pack MD, Agger R, Crowley MT, Lawless D, Steinman RM. The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies. J Exp Med. 1990 May 1;171(5):1753-71. PMID: 2185332 3: McMenamin PG. Dendritic cells and macrophages in the uveal tract of the normal mouse eye. Br J Ophthalmol. 1999 May;83(5):598-604. PMID: 10216062 4: Pimorady-Esfahani A, Grounds MD, McMenamin PG. Macrophages and dendritic cells in normal and regenerating murine skeletal muscle. Muscle Nerve. 1997 Feb;20(2):158-66. PMID: 9040653 5: Kelsall BL, Strober W. Distinct populations of dendritic cells are present in the subepithelial dome and T cell regions of the murine Peyer's patch. J Exp Med. 1996 Jan 1;183(1):237-47. PMID: 8551227 6: Dahlen E, Dawe K, Ohlsson L, Hedlund G. Dendritic cells and macrophages are the first and major producers of TNF-alpha in pancreatic islets in the nonobese diabetic mouse. J Immunol. 1998 Apr 1;160(7):3585-93. PMID: 9531322 7: Leenen PJ, Radosevic K, Voerman JS, Salomon B, van Rooijen N, Klatzmann D, van Ewijk W. Heterogeneity of mouse spleen dendritic cells: in vivo phagocytic activity, expression of macrophage markers, and subpopulation turnover. J Immunol. 1998 Mar 1;160(5):2166-73. PMID: 9498754 8: Gonzalez-Juarrero M, Orme IM. Characterization of murine lung dendritic cells infected with Mycobacterium tuberculosis. Infect Immun. 2001 Feb;69(2):1127-33. PMID: 11160010 9: Hamada H, Hiroi T, Nishiyama Y, Takahashi H, Masunaga Y, Hachimura S, Kaminogawa S, Takahashi-Iwanaga H, Iwanaga T, Kiyono H, Yamamoto H, Ishikawa H. Identification of multiple isolated lymphoid follicles on the antimesenteric wall of the mouse small intestine. J Immunol. 2002 Jan 1;168(1):57-64. PMID: 11751946 10: Bjorck P. Dendritic cells exposed to herpes simplex virus in vivo do not produce IFN-alpha after rechallenge with virus in vitro and exhibit decreased T cell alloreactivity. J Immunol. 2004 May 1;172(9):5396-404. PMID: 15100280 Please note that most of these appear to be available freely via PubMed. Reference 1 has some great pictures of staining in the liver and in spleen with N418 and other antibodies such as F4/80 and FA-11 etc. I hope this advice will be useful. Please do not hesitate to contact me if you still experience problems with those recommendations, if you purchased the antibody in the last 90 days I would be happy to offer you a refund,

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    Answer

    Thank you for your enquiry. The protocol quoted in the link you kindly provided does not seem to mention our antibodies but antibodies from other companies. In our experience the secondary ab6782 does not give background problems and does not need to be pre-absorbed. If you experience any problems or need further advice regarding your IHC protocol please do not hesitate to contact me, I would be happy to look at your full protocol,

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    Answer

    This antibody is not recommended for Armenian hamster IgG.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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