Rabbit Anti-Syrian Hamster IgG H&L (Biotin) (ab6782)

Submit an Abreview Q&A (3)Specific References (3)

Overview

  • Product name
    Rabbit Anti-Syrian Hamster IgG H&L (Biotin)
    See all IgG secondary antibodies
  • Host species
    Rabbit
  • Target species
    Syrian hamster
  • Tested applications
    Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details
  • Immunogen

    hamster IgG whole molecule

  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule.
  • Conjugation notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
ICC/IF
WB
  • Application notes
    immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency

    • Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit polyclonal Secondary Antibody to Syrian Hamster IgG - H&L (Biotin) (ab6782)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit polyclonal Secondary Antibody to Syrian Hamster IgG - H&L (Biotin) (ab6782)
      Ab6782 was used at dilution 1/800 with the primary antibody ab11936 in IHC-P. See the review on ab11936.

    References

    This product has been referenced in:
    • Alymova IV  et al. A novel cytotoxic sequence contributes to influenza A viral protein PB1-F2 pathogenicity and predisposition to secondary bacterial infection. J Virol 88:503-15 (2014). Read more (PubMed: 24173220) »
    • Guo Q  et al. Mouse lymphatic endothelial cell targeted probes: anti-LYVE-1 antibody-based magnetic nanoparticles. Int J Nanomedicine 8:2273-84 (2013). IHC-P . Read more (PubMed: 23818783) »

    See all 3 Publications for this product

    Publishing research using ab6782? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    3 reviews or Q&A

    10
    Votes: Highest First

    Following our standard protocol for IHC, I could not detect specific staining for CD11c in spleen and ear (dermis) sections. A staining with anti MHC II that I performed in parallel worked just fine. Could you please suggest me how to improve my proto...

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    I apologise for the delay in responding to you, I have at last received feedback from the laboratory that tested ab33483 and include their response below: I have had a look through your customers protocol and note that it differs somewhat from our r...

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    I found this information on the web: http://www.histosearch.com/histonet/Oct04/Re.HistonetBackgroundwithC.html where it is recommended to pre-absorb the secondary detecting a hamster monoclonal to CD11c. I bought both antibodies from you, ple...

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    Thank you for your enquiry. The protocol quoted in the link you kindly provided does not seem to mention our antibodies but antibodies from other companies. In our experience the secondary ab6782 does not give background problems and does not need ...

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    Is this Ab appropriate as a labeling secondary Ab (for IHC) to an Armenian hamster IgG, group 3, lamda (anti-mouse CD51 monoclonal antibody)

    This antibody is not recommended for Armenian hamster IgG.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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