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Are your secondary antibodies made against hamster IgG made against Armenian hamster IgG? (e.g. #66783, 6892, 7146)? Approximately what dilution of them would you recommend for an ELISA assay? Are there any pros or cons to rabbit vs. goat? Thanks, K. Spindler
Asked on Jun 28 2001
These antibodies are made against Golden Syrian hamster. The ELISA data suggest the following dilutions: ab6783 is 1:4000 - 1:20,000, ab6892 is 1:15,000 -1:65,000,and ab7146 is 1:4,000 - 1:20,000. There should be no difference in the hosts - it simply provides a choice. However, goat host has a minimal cross-reactivity and rabbit has anti-F(ab')2. We will soon have antisera and IgG fractions to Armenian hamster and are working on affinity purified antisera, but that will take some months.
Neil HaywardAbcam Scientific Support
Answered on Jun 29 2001