Anti-RABEP2 antibody (ab254907)
Key features and details
- Rabbit polyclonal to RABEP2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RABEP2 antibody
See all RABEP2 primary antibodies -
Description
Rabbit polyclonal to RABEP2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Orangutan -
Immunogen
Recombinant fragment corresponding to Human RABEP2 aa 150-300.
Database link: Q9H5N1 -
Positive control
- IHC-P: Human urinary bladder tissue. ICC/IF: MCF7 cells. WB: RT-4 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab254907 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
|
WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 26 kDa.
|
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 26 kDa. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Plays a role in membrane trafficking and in homotypic early endosome fusion. -
Sequence similarities
Belongs to the rabaptin family. -
Cellular localization
Cytoplasm. Early endosome. - Information by UniProt
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Database links
- Entrez Gene: 79874 Human
- Omim: 611869 Human
- SwissProt: Q9H5N1 Human
- Unigene: 555978 Human
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Alternative names
- FLJ23282 antibody
- FRA antibody
- Rab GTPase binding effector protein 2 antibody
see all
Images
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PFA fixed, Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling RABEP2 using ab254907 at 4µg/ml (green) in ICC/IF.
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Paraffin-embedded human urinary bladder tissue stained for RABEP2 using ab254907 at 1/200 dilution in immunohistochemical analysis.
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Lane 2 : Anti-RABEP2 antibody (ab254907) at 0.4 µg/ml
Lane 1 : DNA Ladder
Lane 2 : RT4 cell lysate
Predicted band size: 26 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab254907 has not yet been referenced specifically in any publications.