Key features and details
- Rabbit polyclonal to RABGAP1L/HHL
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RABGAP1L/HHL antibody
DescriptionRabbit polyclonal to RABGAP1L/HHL
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human RABGAP1L/HHL aa 11-262.
Database link: B7ZAP0
- IHC-P: Human liver cancer and gastric cancer tissues. WB: MCF7, K562 and HepG2 whole cell lysates; Mouse stomach, heart, brain and liver lysates.
This product was previously labelled as RABGAP1L
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
- HepG2 whole cell lysate (ab166833)
- Mouse stomach normal tissue lysate - total protein (ab29256)
- Mouse liver tissue lysate - total protein (ab29301)
- MCF7 whole cell lysate (ab29537)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse heart normal tissue lysate - total protein (ab30291)
- MCF7 whole cell lysate (ab3871)
- Mouse brain tissue lysate - total protein (ab4022)
- K562 whole cell lysate (ab7911)
Our Abpromise guarantee covers the use of ab229956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/2000 - 1/5000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).|
RelevanceRABGAP1L contains one PID domain and one Rab-GAP TBC domain. It is up-regulated in esophageal squamous cell carcinomas. Expression is strongly inhibited in the medial septum and hippocampus brain regions of some Alzheimer disease patients. There are nine named isoforms.
- Expressed in hematopoietic cells, heart, liver (HLL) antibody
- HHL antibody
- RAB GTPase activating protein 1 like antibody
Paraffin-embedded human liver cancer tissue stained for RABGAP1L/HHL using ab229956 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human gastric cancer tissue stained for RABGAP1L/HHL using ab229956 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-RABGAP1L/HHL antibody (ab229956) at 1/2000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : Mouse stomach lysate
Lane 5 : Mouse heart lysate
Lane 6 : Mouse brain lysate
Lane 7 : Mouse liver lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
ab229956 has not yet been referenced specifically in any publications.