Overview

  • Product name
    Anti-Rabies Virus antibody [1C5]
    See all Rabies Virus primary antibodies
  • Description
    Mouse monoclonal [1C5] to Rabies Virus
  • Host species
    Mouse
  • Specificity
    This antibody reacts with a Rabies Virus glycoprotein and neutralizes more than 20 different strains from four sero-groups including CVS, Lagosbat, Mokola,and Duwenhage.
  • Tested applications
    Suitable for: ELISA, ICC/IF, IHC-P, IHC-Frmore details
  • Immunogen

    Purified rabies virus

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    1C5
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1.5e+006.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance
    Rabies virus (Neurotropic lyssavirus) is a member of the Rhabdoviridae family. Rabies is a single stranded, neurotropic, negative sense RNA virus which encodes 5 proteins: a glycoprotein, a nucleoprotein, and three others. The mature virus has a bullet shape, a protein coat, and a lipid envelope. The outer surface of the virus is covered with thumblike glycoprotein projections 5-10 nm long and 3 nm in diameter. The virus averages approximately 780 nm in length. Virus infectivity is destroyed by lipid solvents. Rabies virus is a very successful virus, with a very wide range of hosts. It causes an acute, central nervous system infection, characterized by CNS irritation, followed by paralysis and death. Approximately 50,000 human deaths each year are caused by rabies.

References

ab1002 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for kindly providing this information.

In this case, I would be beneficial to the investigations in this case if you could also confirm the following:

1. Please confirm the volume of antibody and volume of reaction mix used in each case.

2. Could you confirm if a sample of the antibodies have been observed under the fluorescence microscope to see if they are fluorescing?

3. Have the IHC experiments been optimized?

Thank you for your continued cooperation. I appreciate your time and look forward to hearing from you with the requested information.

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Answer

Thank you for contacting us again.



ab1002 Anti-Rabies Virus antibody looks very promising as it has been established for the use in IHC-P and should detect a wide range of rabies strains. As a starting dilution the customer can try a 5 ug/ml dilution incubated overnight at 4C. However, it may be that the actual optimum dilution may be lower or higher, but this depends on her samples. Another point is that I would strongly recommend a heat induced Antigen Retrieval if her slides are fixed with formalin.


https://www.abcam.com/index.html?datasheet=1002 (or use the following: https://www.abcam.com/index.html?datasheet=1002).


A matching secondary antibody would be Goat polyclonal Secondary Antibody to Mouse IgG2a - heavy chain (Biotin), pre-adsorbed (ab98696)


https://www.abcam.com/index.html?datasheet=98696 (or use the following: https://www.abcam.com/index.html?datasheet=98696).


To be fair, I am not the biggest fan of ready- to- use solution (andmost of my colleagues here a neither), for the following reason: most of all antibodies need optimization, may it be the blocking step, the retrieval time or method, the antibody incubation buffer etc. With ready-mix solutions it almost impossible to adjust the experiment settings. Ready mix solution make sense if the antibody is used in a high -throughput lab (like 20 slides at once with a fixed protocol), but not if you have to "learn" IHC-P and have the freedom to adjust the protocol fore the perfect results. In addition, it's cheaper ;)


Of course, once the protocol is optimized, using ready mixed solution are a very time efficient and make life easier, but I wouldn't use them as a beginner.


All the buffers your customer needs (see attachment) are listed in our very detailed and easy to follow step-by-step our protocol. However, none of my advice is written in stone, so if your customer prefers the ready mix solutions, we are more than happy to sell them to her.


Something we cannot provide are the reagents for the de-paraffinization, I am afraid, but a company like Sigma should be able to help with that.


Pease sent the customer our protocol and my advice. I am more than happy to assist her to get her experiments running. And another thing: she shouldn't be shy to ask any question she may have about the protocol!

I hope this more information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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https://www.abcam.com/abreviews

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Answer

Thank you for your message. I am sorry we do not sell a biotinylated Rabies virus antibody. I can confirm the ab102864  EasyLink Biotin (type A) Conjugation Kit  will be suitable to conjugate ab1002 for your experiments. I hope this will be helpful. If you have any further questions,please do not hesitate to contact us.

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Answer

Thank you for your inquiry. The concentration of this antibody is 1 mg/ml. I'm not sure what application your customer is using, but I would assume that it is IHC.  The customer can try a 5 ug/ml dilution incubated overnight at 4C. I hope this information helps.  Please contact us with any other questions.

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Answer

Thank you for your enquiry. We do not have a recommended dilution for IHC, but the titer is quite high as you can see on the product datasheet: 1/1,500,000. We would suggest testing it at 1/100 and make sure that the customer applies antigen retrieval if he/she uses formalin-fixed paraffin embedded sections. The antibody does not require any specific procedure so for details on IHC and antigen retrieval, please click on the Resources section on the top panel of the Abcam homepage, you might find some useful information there. If you need anything further or any help then please let us know.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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