Recombinant
RabMAb

Anti-RABL3 antibody [EPR16709] - C-terminal (ab196024)

Overview

  • Product name
    Anti-RABL3 antibody [EPR16709] - C-terminal
    See all RABL3 primary antibodies
  • Description
    Rabbit monoclonal [EPR16709] to RABL3 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human RABL3 aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q5HYI8

  • Positive control
    • MCF7, K562 and 293 cell lysates. Human renal adenocarcinoma and rat liver tissues. A549 and MCF7 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab196024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

Images

  • Anti-RABL3 antibody [EPR16709] - C-terminal (ab196024) at 1/10000 dilution + MCF7 cell lysate at 20 µg

    Secondary
    Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 26 kDa
    Observed band size: 26 kDa

  • All lanes : Anti-RABL3 antibody [EPR16709] - C-terminal (ab196024) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 26 kDa
    Observed band size: 26 kDa

  • Immunohistochemical analysis of paraffin-embedded, human renal adenocarcinoma tissue labeling RABL3 with ab196024 at a 1/100 dilution. Counter stained with hematoxylin. As secondary antibody goat anti-rabbit IgG H&L (HRP) ab97051 was used at a 1/500. In blue DAPI staining.

  • Immunohistochemical analysis of paraffin-embedded, rat liver tissue labeling RABL3 with ab196024 at a 1/100 dilution. Counter stained with hematoxylin. As secondary antibody goat anti-rabbit IgG H&L (HRP) ab97051 was used at a 1/500. In blue DAPI staining.

  • Immunofluorescence analysis of paraformaldehyde-fixed A549 cells labeling RABL3 with ab196024 at a 1/100 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/500. In blue DAPI staining.

  • Flow cytometry analysis of paraformaldehyde-fixed MCF7 cells labeling RABL3 with ab196024 at a 1/100 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, isotype control rabbit IgG (black) and unlabeled control cell without incubation with primary antibody and secondary antibody (Blue).

References

ab196024 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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