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    rabphilin-3a-antibody-ab3338.pdf

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Anti-Rabphilin 3A antibody (ab3338)

  • Datasheet
  • SDS
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Western blot - Anti-Rabphilin 3A antibody (ab3338)

    Key features and details

    • Rabbit polyclonal to Rabphilin 3A
    • Suitable for: WB
    • Reacts with: Rat
    • Isotype: IgG

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    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-Rabphilin 3A antibody
      See all Rabphilin 3A primary antibodies
    • Description

      Rabbit polyclonal to Rabphilin 3A
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Rat
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Rat Rabphilin 3A aa 1-18.
      Sequence:

      MTDTVVNRWMYPGDGPLQ


      (Peptide available as ab4954)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • Rat brain whole protein extract.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Constituents: 0.1% BSA, 99% PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurotransmission
      • Secretory Vesicles
      • Rabs
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Synapse marker

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab3338 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Rat
    All applications
    Mouse
    Application Abreviews Notes
    WB (1)
    Use a concentration of 1 µg/ml.

    By Western blot, this antibody detects an ~75 kDa protein representing Rabphilin 3A from rat brain whole protein extract.

    Notes
    WB
    Use a concentration of 1 µg/ml.

    By Western blot, this antibody detects an ~75 kDa protein representing Rabphilin 3A from rat brain whole protein extract.

    Target

    • Function

      Protein transport. Probably involved with Ras-related protein Rab-3A in synaptic vesicle traffic and/or synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.
    • Sequence similarities

      Contains 2 C2 domains.
      Contains 1 FYVE-type zinc finger.
      Contains 1 RabBD (Rab-binding) domain.
    • Cellular localization

      Cell junction > synapse.
    • Target information above from: UniProt accession Q9Y2J0 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 19894 Mouse
      • Entrez Gene: 171039 Rat
      • SwissProt: P47708 Mouse
      • SwissProt: P47709 Rat
      • Unigene: 181166 Mouse
      • Unigene: 10976 Rat
      • Alternative names

        • Exophilin 1 antibody
        • Exophilin-1 antibody
        • Exophilin1 antibody
        • KIAA0985 antibody
        • Rabphilin 3A homolog antibody
        • Rabphilin-3A antibody
        • Rabphilin3A antibody
        • RP3A antibody
        • RP3A_HUMAN antibody
        • Rph 3a antibody
        • Rph3a antibody
        see all

      Images

      • Western blot - Anti-Rabphilin 3A antibody (ab3338)
        Western blot - Anti-Rabphilin 3A antibody (ab3338)

        Western blot detection of Rabphilin 3A in rat brain extract using ab3338.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab3338? Please let us know so that we can cite the reference in this datasheet.

      ab3338 has been referenced in 1 publication.

      • Xu XQ  et al. S1PR1 mediates anti-apoptotic/pro-proliferative processes in human acute myeloid leukemia cells. Mol Med Rep 14:3369-75 (2016). PubMed: 27572094

      Customer reviews and Q&As

      Show All Reviews Q&A
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      Western blot abreview for Anti-Rabphilin 3A antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      5 µg
      Gel Running Conditions
      Non-reduced Denaturing
      Sample
      Rat Tissue lysate - whole (hippocampus tissue lysate)
      Specification
      hippocampus tissue lysate
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 07 2014

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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