Product nameAnti-Rabphilin 3A antibody
See all Rabphilin 3A primary antibodies
DescriptionRabbit polyclonal to Rabphilin 3A
Specificityab59259 detects endogenous levels of total Rabphilin 3A protein.
Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic non-phosphopeptide derived from human Rabphilin 3A around the phosphorylation site of serine 234 (R-A-SP-E-A).
- IHC-P: human brain tissue WB: HeLa cell extracts treated with TNF alpha (20ng/ml, 2mins)
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab59259 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Our Abpromise guarantee covers the use of ab59259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).|
FunctionProtein transport. Probably involved with Ras-related protein Rab-3A in synaptic vesicle traffic and/or synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.
Sequence similaritiesContains 2 C2 domains.
Contains 1 FYVE-type zinc finger.
Contains 1 RabBD (Rab-binding) domain.
Cellular localizationCell junction > synapse.
- Information by UniProt
- Exophilin 1 antibody
- Exophilin-1 antibody
- Exophilin1 antibody
All lanes : Anti-Rabphilin 3A antibody (ab59259) at 1/500 dilution
Lane 1 : HeLa cell extracts treated
with TNF alpha (20ng/ml, 2mins)
Lane 2 : HeLa cell extracts treated
with TNF alpha (20ng/ml, 2mins) with immunising peptide
Predicted band size: 77 kDa
Observed band size: 77 kDa
ab59259 at 1/50 dilution staining Rabphilin 3A in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
This product has been referenced in:
- Song SY et al. Environmental Enrichment Upregulates Striatal Synaptic Vesicle-Associated Proteins and Improves Motor Function. Front Neurol 9:465 (2018). Read more (PubMed: 30061854) »
- Kang S et al. Effects of a Dehydroevodiamine-Derivative on Synaptic Destabilization and Memory Impairment in the 5xFAD, Alzheimer's Disease Mouse Model. Front Behav Neurosci 12:273 (2018). Read more (PubMed: 30483077) »