Overview

  • Product name

    Anti-RAC1 + Cdc42 (phospho S71) antibody
    See all RAC1 + Cdc42 primary antibodies
  • Description

    Rabbit polyclonal to RAC1 + Cdc42 (phospho S71)
  • Host species

    Rabbit
  • Specificity

    ab129743 detects endogenous levels of Rac1 only when phosphorylated at serine 71.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human RAC1 + Cdc42.

  • Positive control

    • Extract from 293 cells treated with PMA.

Properties

Applications

Our Abpromise guarantee covers the use of ab129743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 21 kDa.

Target

  • Relevance

    Cdc42/Rac belongs to the superfamily of small GTPases that are structurally linked to the proto-oncogene product p21ras and are important for the control of cell growth and differentiation as well as for intracellular organization. Cdc42/Rac is an important upstream regulator of the protein kinase cascade that controls the SAPK/JNK and p38 activity. Recent data also suggest that constitutive active forms of Cdc42 can induce apoptosis through a mechanism requiring signaling through SAPK/JNK.
  • Cellular localization

    Cell membrane; Lipid anchor; Cytoplasmic side.
  • Database links

  • Alternative names

    • CDC42Hs antibody
    • Cell division cycle 42 antibody
    • G25K antibody
    • MIG5 antibody
    • Migration inducing gene 5 antibody
    • p21 Rac1 antibody
    • Ras like protein TC25 antibody
    • Small GTP binding protein Cdc42 antibody
    • TC25 antibody
    see all

Images

  • All lanes : Anti-RAC1 + Cdc42 (phospho S71) antibody (ab129743) at 1/500 dilution

    Lane 1 : Extract from 293 cells, untreated
    Lane 2 : Extract from 293 cells
    treated with PMA

    Predicted band size: 21 kDa

References

ab129743 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Drosophila melanogaster Cell lysate - whole cell (S2 cells lysate)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
50 µg
Treatment
DMSO for control and 20µM, 50µm, 100µM Forskolin for 30 min
Specification
S2 cells lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 11 2018

Answer

ab129743 is specific to phosphorylated Rac1 (Ser71), it does not react with Rac2, Rac3, Cdc 42, Rho A/B/C, based on its specific affinity purification method. Thus, we guarantee the antibody to be specific to Rac1 phosphorylated on Ser71.

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