Overview

  • Product name

    Anti-RAC1 + Cdc42 (phospho S71) antibody
    See all RAC1 + Cdc42 primary antibodies
  • Description

    Rabbit polyclonal to RAC1 + Cdc42 (phospho S71)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human RAC1 + Cdc42 aa 60-75 (phospho S71) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    GQEDYDRLRPLSYPQT


    Database link: P63000

  • Positive control

    • Human colon cancer tissue; FHC cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab203884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.

When using a fluorescent probe the recommended dilution is 1/50 – 1/200.

WB 1/100 - 1/1000. Predicted molecular weight: 21 kDa.
ICC/IF 1/100.

Target

  • Relevance

    Cdc42/Rac belongs to the superfamily of small GTPases that are structurally linked to the proto-oncogene product p21ras and are important for the control of cell growth and differentiation as well as for intracellular organization. Cdc42/Rac is an important upstream regulator of the protein kinase cascade that controls the SAPK/JNK and p38 activity. Recent data also suggest that constitutive active forms of Cdc42 can induce apoptosis through a mechanism requiring signaling through SAPK/JNK.
  • Cellular localization

    Cell membrane; Lipid anchor; Cytoplasmic side.
  • Database links

  • Alternative names

    • CDC42Hs antibody
    • Cell division cycle 42 antibody
    • G25K antibody
    • MIG5 antibody
    • Migration inducing gene 5 antibody
    • p21 Rac1 antibody
    • Ras like protein TC25 antibody
    • Small GTP binding protein Cdc42 antibody
    • TC25 antibody
    see all

Images

  • Immunofluorescent analysis of FHC cells labeling Rac1 with ab203884 at 1/100 dilution followed by Goat Anti-Rabbit IgG (H + L)-Cy3.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon cancer tissue labeling Rac1 with ab203884 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

References

ab203884 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Drosophila melanogaster Cell lysate - whole cell (S2 cells)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
50 µg
Treatment
DMSO for control and 20-50-100µM Forskolin
Specification
S2 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 11 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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