• Product name

    Anti-Rac1 antibody - C-terminal
    See all Rac1 primary antibodies
  • Description

    Rabbit polyclonal to Rac1 - C-terminal
  • Host species

  • Specificity

    ab187108 is recommended for detection of Rac 1 and, to a lesser extent, Rac 3 of mouse, rat and human origin, including equine, canine, bovine, porcine and avian. There is no cross reactivity with Rac 2.
  • Tested applications

    Suitable for: IP, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Horse, Chicken, Cow, Dog, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to Human Rac1 (C terminal).
    Database link: P63000

  • Positive control

    • Non-transfected 293 and Mouse Rac 1 transfected 293 whole cell lysates.



Our Abpromise guarantee covers the use of ab187108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-4 µg/mg of lysate.
WB 1/100 - 1/1000. Predicted molecular weight: 21 kDa.
ICC/IF 1/50 - 1/500.


  • Function

    Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
    Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.
  • Tissue specificity

    Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Rho family.
  • Domain

    The effector region mediates interaction with DEF6.
  • Post-translational

    AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
  • Cellular localization

    Cell membrane. Melanosome. Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Form

    Rac1 has high sequence homology with other Rac proteins. Therefore some antibodies may show cross-reactivity.
  • Alternative names

    • Cell migration inducing gene 5 protein antibody
    • Cell migration-inducing gene 5 protein antibody
    • MGC111543 antibody
    • MIG5 antibody
    • Migration inducing gene 5 antibody
    • Migration inducing protein 5 antibody
    • p21 Rac1 antibody
    • p21-Rac1 antibody
    • Rac 1 antibody
    • RAC1 antibody
    • RAC1_HUMAN antibody
    • Ras like protein TC25 antibody
    • Ras related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) antibody
    • Ras-like protein TC25 antibody
    • Ras-related C3 botulinum toxin substrate 1 antibody
    • Rho family small GTP binding protein Rac1 antibody
    • TC 25 antibody
    • TC25 antibody
    see all


  • All lanes : Anti-Rac1 antibody - C-terminal (ab187108) at 1/100 dilution

    Lane 1 : Non-transfected 293 cell lysate
    Lane 2 : Mouse Rac1 transfected 293 cell lysate
    Lane 3 : Rac 1 expression in whole cell lysate

    Predicted band size: 21 kDa


ab187108 has not yet been referenced specifically in any publications.

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