Overview

  • Product name
    Rac2 Activation Assay Kit
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Species reactivity
    Reacts with: Mouse, Human
  • Product overview

    Rac2 Activation Assay Kit (ab211162) uses PAK1 PBD Agarose beads to selectively isolate and pull-down the active form of Rac from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Rac is detected by western blot analysis using an anti-Rac2 specific polyclonal antibody, which reacts with the human and mouse protein.


    Features: 1) non radioactive assay format; 2) fast results: 1 hour assay plus electrophoresis/blotting time; 3) includes Cdc42 positive control; 4) pink colored agarose beads for easy identification during washing and aspiration steps.

  • Notes

    Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways. 

    Rac2 (Ras-Related C3 Botulinum Toxin Substrate 2), a 21 kDa protein, belongs to the family of Rho GTPases and regulates a variety of biological response pathways that include secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Like other small GTPases, Rac2 regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Rac2 binds specifically to the p21-binding domain (PBD) of p21-activated protein kinase (PAK) to control downstream signaling cascades.

    Diseases associated with Rac2 include neutrophil immunodeficiency syndrome and chronic granulomatous disease.

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 20 tests 5 tests
    ab211177 - PAK1 PBD Agarose Beads 1 x 800µl 1 x 200µl
    100X GTPyS 1 x 50µl 1 x 20µl
    100X GDP 1 x 50µl 1 x 20µl
    5X Assay/Lysis Buffer 1 x 30ml 4 x 2ml
    Anti-rac2 Rabbit polyclonal antibody 1 x 40µl 1 x 10µl
    Rac2 immunoblot Positive Control 1 x 100µl 1 x 100µl
  • Research areas
  • Function
    Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase.
  • Tissue specificity
    Hematopoietic specific.
  • Involvement in disease
    Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome (NEUID) [MIM:608203].
  • Sequence similarities
    Belongs to the small GTPase superfamily. Rho family.
  • Cellular localization
    Cytoplasm. Membrane-associated when activated.
  • Information by UniProt
  • Alternative names
    • EN 7
    • EN-7
    • EN7
    • GX
    • HSPC 022
    • HSPC022
    • p21 Rac 2
    • p21 Rac2
    • p21-Rac2
    • p21Rac2
    • RAC 2
    • Rac2
    • RAC2_HUMAN
    • Ras related C3 botulinum toxin substrate 2
    • Ras related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2
    • Ras related C3 botulinum toxin substrate 3
    • Ras related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac2)
    • Ras-related C3 botulinum toxin substrate 2
    • Rho family small GTP binding protein Rac 2
    • Rho family small GTP binding protein Rac2
    • Small G protein
    see all
  • Database links

Associated products

Images

  • Rac2 positive control (recombinant human Rac2 from E. coli, partially purified) detected by immunoblot.

  • Dot blot that demonstrates specificity of anti-Rac2 rabbit antibody.

Protocols

References

ab211162 has not yet been referenced specifically in any publications.

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