Product nameRac2 Activation Assay Kit
Sample typeTissue, Adherent cells, Suspension cells
Species reactivityReacts with: Mouse, Human
Rac2 Activation Assay Kit (ab211162) uses PAK1 PBD Agarose beads to selectively isolate and pull-down the active form of Rac from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Rac is detected by western blot analysis using an anti-Rac2 specific polyclonal antibody, which reacts with the human and mouse protein.
Features: 1) non radioactive assay format; 2) fast results: 1 hour assay plus electrophoresis/blotting time; 3) includes Cdc42 positive control; 4) pink colored agarose beads for easy identification during washing and aspiration steps.
Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways.
Rac2 (Ras-Related C3 Botulinum Toxin Substrate 2), a 21 kDa protein, belongs to the family of Rho GTPases and regulates a variety of biological response pathways that include secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Like other small GTPases, Rac2 regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Rac2 binds specifically to the p21-binding domain (PBD) of p21-activated protein kinase (PAK) to control downstream signaling cascades.
Diseases associated with Rac2 include neutrophil immunodeficiency syndrome and chronic granulomatous disease.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 20 tests 5 tests ab211177 - PAK1 PBD Agarose Beads 1 x 800µl 1 x 200µl 100X GTPyS 1 x 50µl 1 x 20µl 100X GDP 1 x 50µl 1 x 20µl 5X Assay/Lysis Buffer 1 x 30ml 4 x 2ml Anti-rac2 Rabbit polyclonal antibody 1 x 40µl 1 x 10µl Rac2 immunoblot Positive Control 1 x 100µl 1 x 100µl
FunctionPlasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase.
Tissue specificityHematopoietic specific.
Involvement in diseaseDefects in RAC2 are the cause of neutrophil immunodeficiency syndrome (NEUID) [MIM:608203].
Sequence similaritiesBelongs to the small GTPase superfamily. Rho family.
Cellular localizationCytoplasm. Membrane-associated when activated.
- Information by UniProt
- EN 7
ab211162 has not yet been referenced specifically in any publications.