Overview

  • Product name

  • Description

    Rabbit polyclonal to RAC2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Drosophila melanogaster, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-189 of Human RAC2 (UniProt: P15153).

  • Positive control

    • Human NCI-N87 xenograft tissue; MOLT4 and Raji cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 21 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Function

    Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase.
  • Tissue specificity

    Hematopoietic specific.
  • Involvement in disease

    Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome (NEUID) [MIM:608203].
  • Sequence similarities

    Belongs to the small GTPase superfamily. Rho family.
  • Cellular localization

    Cytoplasm. Membrane-associated when activated.
  • Information by UniProt
  • Database links

  • Form

    Rac2 has high sequence homology with other Rac proteins. Therefore some antibodies may show cross-reactivity.
  • Alternative names

    • EN 7 antibody
    • EN-7 antibody
    • EN7 antibody
    • GX antibody
    • HSPC 022 antibody
    • HSPC022 antibody
    • p21 Rac 2 antibody
    • p21 Rac2 antibody
    • p21-Rac2 antibody
    • p21Rac2 antibody
    • RAC 2 antibody
    • Rac2 antibody
    • RAC2_HUMAN antibody
    • Ras related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2 antibody
    • Ras related C3 botulinum toxin substrate 2 antibody
    • Ras related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac2) antibody
    • Ras related C3 botulinum toxin substrate 3 antibody
    • Ras-related C3 botulinum toxin substrate 2 antibody
    • Rho family small GTP binding protein Rac 2 antibody
    • Rho family small GTP binding protein Rac2 antibody
    • Small G protein antibody
    see all

Images

  • Anti-RAC2 antibody (ab154711) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 21 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human NCI-N87 xenograft tissue labeling RAC2 with ab154711 at 1/500 dilution.

References

ab154711 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Dog Tissue lysate - whole (retina)
Gel Running Conditions
Reduced Denaturing (10% Tris Glycine)
Loading amount
50 µg
Specification
retina
Blocking step
Odyssey blocking buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C

Raghavi Sudharsan

Verified customer

Submitted Dec 14 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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