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    rac2-antibody-ab154711.pdf

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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other
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Anti-RAC2 antibody (ab154711)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (2)

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Western blot - Anti-RAC2 antibody (ab154711)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC2 antibody (ab154711)

Key features and details

  • Rabbit polyclonal to RAC2
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
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Recombinant Human RAC2 protein (ab84655)

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Overview

  • Product name

    Anti-RAC2 antibody
    See all RAC2 primary antibodies
  • Description

    Rabbit polyclonal to RAC2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Drosophila melanogaster, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-189 of Human RAC2 (UniProt: P15153).

  • Positive control

    • Human NCI-N87 xenograft tissue; MOLT4 and Raji cell lysates.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • MOLT-4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant Human RAC2 protein (ab84655)
  • Related Products

    • Recombinant Human RAC2 protein (ab78684)
    • Recombinant Human RAC2 protein (ab84655)
    • Recombinant Human RAC2 protein (ab87665)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/500 - 1/3000. Predicted molecular weight: 21 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 21 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Function

    Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase.
  • Tissue specificity

    Hematopoietic specific.
  • Involvement in disease

    Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome (NEUID) [MIM:608203].
  • Sequence similarities

    Belongs to the small GTPase superfamily. Rho family.
  • Cellular localization

    Cytoplasm. Membrane-associated when activated.
  • Target information above from: UniProt accession P15153 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 418044 Chicken
    • Entrez Gene: 327671 Cow
    • Entrez Gene: 38831 Drosophila melanogaster
    • Entrez Gene: 5880 Human
    • Entrez Gene: 19354 Mouse
    • Entrez Gene: 366957 Rat
    • Entrez Gene: 444184 Xenopus laevis
    • Entrez Gene: 496738 Xenopus tropicalis
    • Entrez Gene: 415151 Zebrafish
    • Omim: 602049 Human
    • SwissProt: Q9TU25 Cow
    • SwissProt: P48554 Drosophila melanogaster
    • SwissProt: P15153 Human
    • SwissProt: Q05144 Mouse
    • Unigene: 517601 Human
    • Unigene: 1972 Mouse
    see all
  • Form

    Rac2 has high sequence homology with other Rac proteins. Therefore some antibodies may show cross-reactivity.
  • Alternative names

    • EN 7 antibody
    • EN-7 antibody
    • EN7 antibody
    • GX antibody
    • HSPC 022 antibody
    • HSPC022 antibody
    • p21 Rac 2 antibody
    • p21 Rac2 antibody
    • p21-Rac2 antibody
    • p21Rac2 antibody
    • RAC 2 antibody
    • Rac2 antibody
    • RAC2_HUMAN antibody
    • Ras related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2 antibody
    • Ras related C3 botulinum toxin substrate 2 antibody
    • Ras related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac2) antibody
    • Ras related C3 botulinum toxin substrate 3 antibody
    • Ras-related C3 botulinum toxin substrate 2 antibody
    • Rho family small GTP binding protein Rac 2 antibody
    • Rho family small GTP binding protein Rac2 antibody
    • Small G protein antibody
    see all

Images

  • Western blot - Anti-RAC2 antibody (ab154711)
    Western blot - Anti-RAC2 antibody (ab154711)
    Anti-RAC2 antibody (ab154711) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 21 kDa



    12% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC2 antibody (ab154711)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC2 antibody (ab154711)
    Immunohistochemical analysis of paraffin-embedded Human NCI-N87 xenograft tissue labeling RAC2 with ab154711 at 1/500 dilution.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab154711? Please let us know so that we can cite the reference in this datasheet.

ab154711 has been referenced in 2 publications.

  • Jiang XH  et al. Effect of Grilled Nux Vomica on Differential RNA Expression Profile of Gastrocnemius Muscle and Toll-Like Receptor 4 (TLR-4)/Nuclear Factor kappa B (NF-?B) Signaling in Experimental Autoimmune Myasthenia Gravis Rats. Med Sci Monit 26:e919150 (2020). PubMed: 32052794
  • Xia P  et al. Down-regulation of RAC2 by small interfering RNA restrains the progression of osteosarcoma by suppressing the Wnt signaling pathway. Int J Biol Macromol 137:1221-1231 (2019). PubMed: 31279058

Customer reviews and Q&As

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Western blot abreview for Anti-RAC2 antibody

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Dog Tissue lysate - whole (retina)
Gel Running Conditions
Reduced Denaturing (10% Tris Glycine)
Loading amount
50 µg
Specification
retina
Blocking step
Odyssey blocking buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C
Read More

Raghavi Sudharsan

Verified customer

Submitted Dec 14 2017

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