Anti-RAC2 antibody (ab154711)
Key features and details
- Rabbit polyclonal to RAC2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RAC2 antibody
See all RAC2 primary antibodies -
Description
Rabbit polyclonal to RAC2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Drosophila melanogaster, Zebrafish, Xenopus tropicalis
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-189 of Human RAC2 (UniProt: P15153).
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Positive control
- Human NCI-N87 xenograft tissue; MOLT4 and Raji cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154711 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 21 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 21 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
Target
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Function
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase. -
Tissue specificity
Hematopoietic specific. -
Involvement in disease
Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome (NEUID) [MIM:608203]. -
Sequence similarities
Belongs to the small GTPase superfamily. Rho family. -
Cellular localization
Cytoplasm. Membrane-associated when activated. - Information by UniProt
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Database links
- Entrez Gene: 418044 Chicken
- Entrez Gene: 327671 Cow
- Entrez Gene: 38831 Drosophila melanogaster
- Entrez Gene: 5880 Human
- Entrez Gene: 19354 Mouse
- Entrez Gene: 366957 Rat
- Entrez Gene: 444184 Xenopus laevis
- Entrez Gene: 496738 Xenopus tropicalis
see all -
Form
Rac2 has high sequence homology with other Rac proteins. Therefore some antibodies may show cross-reactivity. -
Alternative names
- EN 7 antibody
- EN-7 antibody
- EN7 antibody
see all
Images
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Western blot - Anti-RAC2 antibody (ab154711)Anti-RAC2 antibody (ab154711) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 21 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC2 antibody (ab154711)Immunohistochemical analysis of paraffin-embedded Human NCI-N87 xenograft tissue labeling RAC2 with ab154711 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab154711 has been referenced in 2 publications.
- Jiang XH et al. Effect of Grilled Nux Vomica on Differential RNA Expression Profile of Gastrocnemius Muscle and Toll-Like Receptor 4 (TLR-4)/Nuclear Factor kappa B (NF-?B) Signaling in Experimental Autoimmune Myasthenia Gravis Rats. Med Sci Monit 26:e919150 (2020). PubMed: 32052794
- Xia P et al. Down-regulation of RAC2 by small interfering RNA restrains the progression of osteosarcoma by suppressing the Wnt signaling pathway. Int J Biol Macromol 137:1221-1231 (2019). PubMed: 31279058