Anti-RAC2 antibody (ab2244)
Key features and details
- Goat polyclonal to RAC2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RAC2 antibody
See all RAC2 primary antibodies -
Description
Goat polyclonal to RAC2 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide:
PQPTRQQKRACSLL
, corresponding to C terminal amino acids 179-192 of Human RAC2. -
Positive control
- Recombinant Human RAC2 protein (ab78684) can be used as a positive control in WB. MOLT4 or liver lysate.
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General notes
Principal Names - RAC2; ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2); EN-7; Ras-related C3 botulinum toxin substrate 2; rho family, small GTP binding protein Rac2; Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP); Gx; HSPC022. Official Gene Symbol - RAC2. GenBank Accession Number – NP_002863. LocusLink ID - 5880.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
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| WB |
Human
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| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 21 kDa.
A 1 hour primary incubation is recommended for this product. |
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| IHC-P |
Use a concentration of 0.3 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In Human Tonsil most cells in the mantle zone and interfollicular area are stained whilst those in the germinal centres remain negative.
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| Notes |
|---|
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WB
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 21 kDa. A 1 hour primary incubation is recommended for this product. |
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IHC-P
Use a concentration of 0.3 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In Human Tonsil most cells in the mantle zone and interfollicular area are stained whilst those in the germinal centres remain negative. |
Target
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Function
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase. -
Tissue specificity
Hematopoietic specific. -
Involvement in disease
Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome (NEUID) [MIM:608203]. -
Sequence similarities
Belongs to the small GTPase superfamily. Rho family. -
Cellular localization
Cytoplasm. Membrane-associated when activated. - Information by UniProt
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Database links
- Entrez Gene: 5880 Human
- Omim: 602049 Human
- SwissProt: P15153 Human
- Unigene: 517601 Human
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Form
Rac2 has high sequence homology with other Rac proteins. Therefore some antibodies may show cross-reactivity. -
Alternative names
- EN 7 antibody
- EN-7 antibody
- EN7 antibody
see all
Images
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Western blot - Anti-RAC2 antibody (ab2244)All lanes : Anti-RAC2 antibody (ab2244) at 0.1 µg/ml
Lane 1 : Human thymus tissue lysate
Lane 2 : Human tonsil tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 21 kDaPrimary incubation was 1 hour. Detected by chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC2 antibody (ab2244)Immunohistochemical analysis of paraffin embedded Human Tonsil tissue section labelling RAC2 with ab2244 (0.3µg/ml). Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Strong staining in the interfollicular area and mantle zone but the majority of cells in germinal centre are negative. B) Higher magnification of interfollicular area showing positive cells.
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Western blot - Anti-RAC2 antibody (ab2244)Anti-RAC2 antibody (ab2244) at 0.3 µg/ml + MOLT4 cell lysate at 35 µg
Predicted band size: 21 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab2244 has been referenced in 8 publications.
- Xia P et al. Down-regulation of RAC2 by small interfering RNA restrains the progression of osteosarcoma by suppressing the Wnt signaling pathway. Int J Biol Macromol 137:1221-1231 (2019). PubMed: 31279058
- Gerasimcik N et al. The Small Rho GTPases Rac1 and Rac2 Are Important for T-Cell Independent Antigen Responses and for Suppressing Switching to IgG2b in Mice. Front Immunol 8:1264 (2017). PubMed: 29056938
- Assani K et al. Human Cystic Fibrosis Macrophages Have Defective Calcium-Dependent Protein Kinase C Activation of the NADPH Oxidase, an Effect Augmented by Burkholderia cenocepacia. J Immunol 198:1985-1994 (2017). PubMed: 28093527
- Sowrirajan B et al. Interleukin-27 Enhances the Potential of Reactive Oxygen Species Generation from Monocyte-derived Macrophages and Dendritic cells by Induction of p47(phox). Sci Rep 7:43441 (2017). PubMed: 28240310
- Hinterseher I et al. Immunohistochemical analysis of the natural killer cell cytotoxicity pathway in human abdominal aortic aneurysms. Int J Mol Sci 16:11196-212 (2015). PubMed: 25993291
- Kawazu M et al. Transforming mutations of RAC guanosine triphosphatases in human cancers. Proc Natl Acad Sci U S A 110:3029-34 (2013). Human . PubMed: 23382236
- Tomilov AA et al. Decreased superoxide production in macrophages of long-lived p66Shc knock-out mice. J Biol Chem 285:1153-65 (2010). WB ; Mouse . PubMed: 19892704
- Ramgolam VS et al. T cell LFA-1 engagement induces HuR-dependent cytokine mRNA stabilization through a Vav-1, Rac1/2, p38MAPK and MKK3 signaling cascade. PLoS One 5:e14450 (2010). WB ; Human . PubMed: 21206905