Overview

  • Product name
    Anti-RAC2 antibody
    See all RAC2 primary antibodies
  • Description
    Goat polyclonal to RAC2
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    PQPTRQQKRACSLL

    , corresponding to C terminal amino acids 179-192 of Human RAC2.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • MOLT4 or liver lysate.
  • General notes
    Principal Names - RAC2; ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2); EN-7; Ras-related C3 botulinum toxin substrate 2; rho family, small GTP binding protein Rac2; Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP); Gx; HSPC022. Official Gene Symbol - RAC2. GenBank Accession Number – NP_002863. LocusLink ID - 5880.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 21 kDa.Can be blocked with Human RAC2 peptide (ab22892).
IHC-P Use a concentration of 0.3 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In Human Tonsil most cells in the mantle zone and interfollicular area are stained whilst those in the germinal centres remain negative.

Target

  • Function
    Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase.
  • Tissue specificity
    Hematopoietic specific.
  • Involvement in disease
    Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome (NEUID) [MIM:608203].
  • Sequence similarities
    Belongs to the small GTPase superfamily. Rho family.
  • Cellular localization
    Cytoplasm. Membrane-associated when activated.
  • Information by UniProt
  • Database links
    • Form
      Rac2 has high sequence homology with other Rac proteins. Therefore some antibodies may show cross-reactivity.
    • Alternative names
      • EN 7 antibody
      • EN-7 antibody
      • EN7 antibody
      • GX antibody
      • HSPC 022 antibody
      • HSPC022 antibody
      • p21 Rac 2 antibody
      • p21 Rac2 antibody
      • p21-Rac2 antibody
      • p21Rac2 antibody
      • RAC 2 antibody
      • Rac2 antibody
      • RAC2_HUMAN antibody
      • Ras related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2 antibody
      • Ras related C3 botulinum toxin substrate 2 antibody
      • Ras related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac2) antibody
      • Ras related C3 botulinum toxin substrate 3 antibody
      • Ras-related C3 botulinum toxin substrate 2 antibody
      • Rho family small GTP binding protein Rac 2 antibody
      • Rho family small GTP binding protein Rac2 antibody
      • Small G protein antibody
      see all

    Images

    • Western blot - Anti-RAC2 antibody (ab2244)
      Western blot - Anti-RAC2 antibody (ab2244)
      All lanes : Anti-RAC2 antibody (ab2244) at 0.1 µg/ml

      Lane 1 : Human thymus tissue lysate
      Lane 2 : Human tonsil tissue lysate

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 21 kDa



      Primary incubation was 1 hour. Detected by chemiluminescence.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC2 antibody (ab2244)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC2 antibody (ab2244)

      Immunohistochemical analysis of paraffin embedded Human Tonsil tissue section labelling RAC2 with ab2244 (0.3µg/ml). Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Strong staining in the interfollicular area and mantle zone but the majority of cells in germinal centre are negative. B) Higher magnification of interfollicular area showing positive cells.

    • Western blot - Anti-RAC2 antibody (ab2244)
      Western blot - Anti-RAC2 antibody (ab2244)
      Anti-RAC2 antibody (ab2244) at 0.3 µg/ml + MOLT4 cell lysate at 35 µg

      Predicted band size: 21 kDa
      Observed band size: 20 kDa (why is the actual band size different from the predicted?)



      Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. 

    References

    This product has been referenced in:
    • Gerasimcik N  et al. The Small Rho GTPases Rac1 and Rac2 Are Important for T-Cell Independent Antigen Responses and for Suppressing Switching to IgG2b in Mice. Front Immunol 8:1264 (2017). Read more (PubMed: 29056938) »
    • Hinterseher I  et al. Immunohistochemical analysis of the natural killer cell cytotoxicity pathway in human abdominal aortic aneurysms. Int J Mol Sci 16:11196-212 (2015). Read more (PubMed: 25993291) »

    See all 5 Publications for this product

    Publishing research using ab2244? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    5 reviews or Q&A

    10
    Votes: Highest First

    Dear Sir/Madam,

    I would like to buy a Rac2 antibody. I saw you sell 2 differents antibodies ab93614 and ab2244 and I would like to know the sequence of the epitop you used for their synthesis.

    Best regards,

    Thank you for your inquiry.

    ab2244 has been produced against the synthetic peptide: PQPTRQQKRACSLL, corresponding to C terminal amino acids 179-192 of Human RAC2.

    ab93614 has been produced using a proprietary sequence peptide derive...

    Read More

    Sehr geehrte Damen und Herren, wir möchten in unserer Arbeitsgruppe beginnen, mit der NADPH-oxidase in Makrophagen (Rattenmakrophagen NR8383, ev. auch humane Monozyten/Makrophagen U937) zu arbeiten und sind auf der Suche nach Antikörpern gegen ihre Bes...

    Read More

    Vielen Dank für Ihre Anfrage. Gegen die genannten NADPH-Oxidase-Komponenten haben wir folgende Antikörper bzw. Proteine in unserem Katalog: Click here (or use the following: https://www.abcam.com/index.html?datasheet=109366). Click here (or use th...

    Read More

    How specific is this antibody for RAC2? Has it been tested for cross-reactivity with RAC1 or RAC3?

    Thank you for your enquiry. This antibody has not been tested for cross-reactivity with RAC1 or RAC3. You can BLAST the sequence to check, but I didn't see any hits for RAC1 or RAC3. If you have any additional questions, please contact us again.

    I have a query re your Rac2 polyclonal antibody (ab2244). I would like to know whether this antibody is absolutely specific for Rac2 and whether it cross-reacts with the very similar proteins Rac1 and/or Rac3. For example, has it been tested for a lack...

    Read More

    Thank you for your question. Our immunogen was PQPTRQQKRACSLL.Cross-reactivity tests have not been done against Rac1 or Rac3, and a BLAST search could only find a significant alignment with RAC2. I hope this information helps you,

    Is this antibody recommended at all for immunohistochemistry? Are there any references available other than the one give in the product data sheet? Thanks!

    This antibody has not been tested for IHC application yet. Should you decide to go ahead and test this antibody, please let us know how you get on, and in return we will forward a USD15/EUR15/GBP10 Amazon gift voucher.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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