Anti-RAC2 antibody (ab2244)

Submit a review Q&A (5)References (7)

Overview

  • Product name
    Anti-RAC2 antibody
    See all RAC2 primary antibodies
  • Description
    Goat polyclonal to RAC2
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    PQPTRQQKRACSLL

    , corresponding to C terminal amino acids 179-192 of Human RAC2.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Recombinant Human RAC2 protein (ab78684) can be used as a positive control in WB. MOLT4 or liver lysate.
  • General notes
    Principal Names - RAC2; ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2); EN-7; Ras-related C3 botulinum toxin substrate 2; rho family, small GTP binding protein Rac2; Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP); Gx; HSPC022. Official Gene Symbol - RAC2. GenBank Accession Number – NP_002863. LocusLink ID - 5880.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 21 kDa.Can be blocked with Human RAC2 peptide (ab22892).
IHC-P Use a concentration of 0.3 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In Human Tonsil most cells in the mantle zone and interfollicular area are stained whilst those in the germinal centres remain negative.

Target

  • Function
    Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase.
  • Tissue specificity
    Hematopoietic specific.
  • Involvement in disease
    Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome (NEUID) [MIM:608203].
  • Sequence similarities
    Belongs to the small GTPase superfamily. Rho family.
  • Cellular localization
    Cytoplasm. Membrane-associated when activated.
  • Information by UniProt
  • Database links
    • Form
      Rac2 has high sequence homology with other Rac proteins. Therefore some antibodies may show cross-reactivity.
    • Alternative names
      • EN 7 antibody
      • EN-7 antibody
      • EN7 antibody
      • GX antibody
      • HSPC 022 antibody
      • HSPC022 antibody
      • p21 Rac 2 antibody
      • p21 Rac2 antibody
      • p21-Rac2 antibody
      • p21Rac2 antibody
      • RAC 2 antibody
      • Rac2 antibody
      • RAC2_HUMAN antibody
      • Ras related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2 antibody
      • Ras related C3 botulinum toxin substrate 2 antibody
      • Ras related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac2) antibody
      • Ras related C3 botulinum toxin substrate 3 antibody
      • Ras-related C3 botulinum toxin substrate 2 antibody
      • Rho family small GTP binding protein Rac 2 antibody
      • Rho family small GTP binding protein Rac2 antibody
      • Small G protein antibody
      see all

    Images

    • Western blot - Anti-RAC2 antibody (ab2244)
      Western blot - Anti-RAC2 antibody (ab2244)
      All lanes : Anti-RAC2 antibody (ab2244) at 0.1 µg/ml

      Lane 1 : Human thymus tissue lysate
      Lane 2 : Human tonsil tissue lysate

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 21 kDa



      Primary incubation was 1 hour. Detected by chemiluminescence.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC2 antibody (ab2244)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC2 antibody (ab2244)

      Immunohistochemical analysis of paraffin embedded Human Tonsil tissue section labelling RAC2 with ab2244 (0.3µg/ml). Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Strong staining in the interfollicular area and mantle zone but the majority of cells in germinal centre are negative. B) Higher magnification of interfollicular area showing positive cells.

    • Western blot - Anti-RAC2 antibody (ab2244)
      Western blot - Anti-RAC2 antibody (ab2244)
      Anti-RAC2 antibody (ab2244) at 0.3 µg/ml + MOLT4 cell lysate at 35 µg

      Predicted band size: 21 kDa
      Observed band size: 20 kDa
      why is the actual band size different from the predicted?



      Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. 

    References

    This product has been referenced in:
    • Gerasimcik N  et al. The Small Rho GTPases Rac1 and Rac2 Are Important for T-Cell Independent Antigen Responses and for Suppressing Switching to IgG2b in Mice. Front Immunol 8:1264 (2017). Read more (PubMed: 29056938) »
    • Assani K  et al. Human Cystic Fibrosis Macrophages Have Defective Calcium-Dependent Protein Kinase C Activation of the NADPH Oxidase, an Effect Augmented by Burkholderia cenocepacia. J Immunol 198:1985-1994 (2017). Read more (PubMed: 28093527) »
    See all 7 Publications for this product

    Publishing research using ab2244? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-5 of 5 Abreviews or Q&A

    Question

    Dear Sir/Madam,

    I would like to buy a Rac2 antibody. I saw you sell 2 differents antibodies ab93614 and ab2244 and I would like to know the sequence of the epitop you used for their synthesis.

    Best regards,

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    Answer

    Thank you for your inquiry.

    ab2244 has been produced against the synthetic peptide: PQPTRQQKRACSLL, corresponding to C terminal amino acids 179-192 of Human RAC2.

    ab93614 has been produced using a proprietary sequence peptide derived from inbetween amino acid 163 to 192 of theC-terminal region of humanRAC2. I am sorry that I can not provide the more detailled sequence, as it is proprietary information of our source of this antibody.

    I hope this information is helpful. Please donot hesitate to contact us again, shouldyou have any concern or further question.

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    Question

    Sehr geehrte Damen und Herren, wir möchten in unserer Arbeitsgruppe beginnen, mit der NADPH-oxidase in Makrophagen (Rattenmakrophagen NR8383, ev. auch humane Monozyten/Makrophagen U937) zu arbeiten und sind auf der Suche nach Antikörpern gegen ihre Bestandteile (p47PHOX, p40PHOX, p67PHOX, P22PHOX, gp91PHOX, Rac1/2, Rho-GDI, …), sowie (falls möglich) die Einzelkomponenten selber (für die Arbeit im zellfreien System). Können Sie uns weiterhelfen? Über ein Angebot würden wir uns freuen.

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    Answer

    Vielen Dank für Ihre Anfrage. Gegen die genannten NADPH-Oxidase-Komponenten haben wir folgende Antikörper bzw. Proteine in unserem Katalog: Click here (or use the following: https://www.abcam.com/index.html?datasheet=109366). Click here (or use the following: https://www.abcam.com/index.html?datasheet=80508). Click here (or use the following: https://www.abcam.com/index.html?datasheet=33186). Click here (or use the following: https://www.abcam.com/index.html?datasheet=2244). Click here (or use the following: https://www.abcam.com/index.html?datasheet=118159). Click here (or use the following: https://www.abcam.com/index.html?datasheet=75941). Click here (or use the following: https://www.abcam.com/index.html?datasheet=115855). Click here (or use the following: https://www.abcam.com/index.html?datasheet=59355). Click here (or use the following: https://www.abcam.com/index.html?datasheet=111855). Click here (or use the following: https://www.abcam.com/index.html?datasheet=795). Click here (or use the following: https://www.abcam.com/index.html?datasheet=88031). Click here (or use the following: https://www.abcam.com/index.html?datasheet=98054). Click here (or use the following: https://www.abcam.com/index.html?datasheet=117065). Click here (or use the following: https://www.abcam.com/index.html?datasheet=114183). Click here (or use the following: https://www.abcam.com/index.html?datasheet=84657). Click here (or use the following: https://www.abcam.com/index.html?datasheet=40471). Click here (or use the following: https://www.abcam.com/index.html?datasheet=89246). Click here (or use the following: https://www.abcam.com/index.html?datasheet=82670). Click here (or use the following: https://www.abcam.com/index.html?datasheet=51014). Click here (or use the following: https://www.abcam.com/index.html?datasheet=84655). Click here (or use the following: https://www.abcam.com/index.html?datasheet=78684). Click here (or use the following: https://www.abcam.com/index.html?datasheet=87665). Vielleicht interessiert Sie in diesem Zusammenhang auch einer unserer Assaykits (NADP/NADPH): Click here (or use the following: https://www.abcam.com/index.html?datasheet=65349). Wir geben alle getesteten Spezies und Anwendungen auf unseren Produktdatenblättern an und aktualisieren diese sobald wir neue Informationen erhalten. Falls sich herausstellt, dass der Antikörper nicht so funktioniert, wie auf dem Datenblatt beschrieben und Sie sich innerhalb von sechs Monaten mit Ihrem Problem an uns wenden, werden wir Ihnen gerne Vorschläge zur Optimierung Ihres Protokolls oder gegebenenfalls einen Ersatz oder eine Gutschrift schicken. Falls der Antikörper in einer bisher nicht getesteten Anwendung oder Spezies verwendet wird (z.B. für ab111855 oder ab115855), qualifizieren Sie sich für unser Testprogramm: Bei diesem Angebot bekommen Sie einen 100%igen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen: https://www.abcam.com/collaborationdiscount. Falls Sie daran interessiert sind oder weitere Fragen haben, kontaktieren Sie bitte unseren wissenschaftlichen Dienst. Die oben genannten Antikörper sind nur eine Auswahl und wir haben noch weitere gegen die entsprechenden Zielproteine erhältlich, die für unterschiedliche Anwendungen getestet und garantiert sind. Bei einer detaillierteren Suche bzw. Beratung bin ich Ihnen gerne weiter behilflich. Gerne senden wir Ihnen auch gerne ein Angebot zu, sobald Sie eine konkrete Auswahl getroffen haben. Ich hoffe, diese Informationen helfen Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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    Question

    How specific is this antibody for RAC2? Has it been tested for cross-reactivity with RAC1 or RAC3?

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    Answer

    Thank you for your enquiry. This antibody has not been tested for cross-reactivity with RAC1 or RAC3. You can BLAST the sequence to check, but I didn't see any hits for RAC1 or RAC3. If you have any additional questions, please contact us again.

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    Question

    I have a query re your Rac2 polyclonal antibody (ab2244). I would like to know whether this antibody is absolutely specific for Rac2 and whether it cross-reacts with the very similar proteins Rac1 and/or Rac3. For example, has it been tested for a lack of reactivity with purified fusion proteins of Rac1/ Rac3? I need a Rac2 antibody that is completely specific, so any reassurance you can give me would be much appreciated before I consider a purchase. Thank you very much, Regards

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    Answer

    Thank you for your question. Our immunogen was PQPTRQQKRACSLL.Cross-reactivity tests have not been done against Rac1 or Rac3, and a BLAST search could only find a significant alignment with RAC2. I hope this information helps you,

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    Question

    Is this antibody recommended at all for immunohistochemistry? Are there any references available other than the one give in the product data sheet? Thanks!

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    Answer

    This antibody has not been tested for IHC application yet. Should you decide to go ahead and test this antibody, please let us know how you get on, and in return we will forward a USD15/EUR15/GBP10 Amazon gift voucher.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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