Anti-RAC2 antibody (ab2244)
Key features and details
- Goat polyclonal to RAC2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RAC2 antibody
See all RAC2 primary antibodies -
Description
Goat polyclonal to RAC2 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide:
PQPTRQQKRACSLL
, corresponding to C terminal amino acids 179-192 of Human RAC2. -
Positive control
- Recombinant Human RAC2 protein (ab78684) can be used as a positive control in WB. MOLT4 or liver lysate.
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General notes
Principal Names - RAC2; ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2); EN-7; Ras-related C3 botulinum toxin substrate 2; rho family, small GTP binding protein Rac2; Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP); Gx; HSPC022. Official Gene Symbol - RAC2. GenBank Accession Number – NP_002863. LocusLink ID - 5880.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 21 kDa.
A 1 hour primary incubation is recommended for this product. |
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IHC-P |
Use a concentration of 0.3 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In Human Tonsil most cells in the mantle zone and interfollicular area are stained whilst those in the germinal centres remain negative.
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Notes |
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WB
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 21 kDa. A 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 0.3 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In Human Tonsil most cells in the mantle zone and interfollicular area are stained whilst those in the germinal centres remain negative. |
Target
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Function
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase. -
Tissue specificity
Hematopoietic specific. -
Involvement in disease
Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome (NEUID) [MIM:608203]. -
Sequence similarities
Belongs to the small GTPase superfamily. Rho family. -
Cellular localization
Cytoplasm. Membrane-associated when activated. - Information by UniProt
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Database links
- Entrez Gene: 5880 Human
- Omim: 602049 Human
- SwissProt: P15153 Human
- Unigene: 517601 Human
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Form
Rac2 has high sequence homology with other Rac proteins. Therefore some antibodies may show cross-reactivity. -
Alternative names
- EN 7 antibody
- EN-7 antibody
- EN7 antibody
see all
Images
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All lanes : Anti-RAC2 antibody (ab2244) at 0.1 µg/ml
Lane 1 : Human thymus tissue lysate
Lane 2 : Human tonsil tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 21 kDaPrimary incubation was 1 hour. Detected by chemiluminescence.
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Immunohistochemical analysis of paraffin embedded Human Tonsil tissue section labelling RAC2 with ab2244 (0.3µg/ml). Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Strong staining in the interfollicular area and mantle zone but the majority of cells in germinal centre are negative. B) Higher magnification of interfollicular area showing positive cells.
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Anti-RAC2 antibody (ab2244) at 0.3 µg/ml + MOLT4 cell lysate at 35 µg
Predicted band size: 21 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Protocols
Datasheets and documents
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Datasheet download
References (11)
ab2244 has been referenced in 11 publications.
- Chen Q et al. The Pyroptosis-Related Risk Genes APOBEC3D, TNFRSF14, and RAC2 Were Used to Evaluate Prognosis and as Tumor Suppressor Genes in Breast Cancer. J Oncol 2022:3625790 (2022). PubMed: 36059808
- Liu Y et al. Proteomic Analysis Revealed the Characteristics of Key Proteins Involved in the Regulation of Inflammatory Response, Leukocyte Transendothelial Migration, Phagocytosis, and Immune Process during Early Lung Blast Injury. Oxid Med Cell Longev 2021:8899274 (2021). PubMed: 34007409
- Xia P et al. Down-regulation of RAC2 by small interfering RNA restrains the progression of osteosarcoma by suppressing the Wnt signaling pathway. Int J Biol Macromol 137:1221-1231 (2019). PubMed: 31279058
- Sawaya AP et al. Mevastatin promotes healing by targeting caveolin-1 to restore EGFR signaling. JCI Insight 4:N/A (2019). PubMed: 31661463
- Gerasimcik N et al. The Small Rho GTPases Rac1 and Rac2 Are Important for T-Cell Independent Antigen Responses and for Suppressing Switching to IgG2b in Mice. Front Immunol 8:1264 (2017). PubMed: 29056938