Recombinant Anti-RAC3 antibody [EPR6680] (ab129062)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6680] to RAC3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-RAC3 antibody [EPR6680]
See all RAC3 primary antibodies -
Description
Rabbit monoclonal [EPR6680] to RAC3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human RAC3 aa 100-200. The exact sequence is proprietary.
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Positive control
- WB: Human brain, Mouse brain, and Rat brain lysates. IHC-P: human cerebrum, mouse cerebrum, and rat cerebrum tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 7.20 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6680 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as cell spreading and the formation of actin-based protusions including lamellipodia and membrane ruffles. Promotes cell adhesion and spreading on fibrinogen in a CIB1 and alpha-IIb/beta3 integrin-mediated manner. -
Tissue specificity
Highest levels in brain, also detected in heart, placenta and pancreas. -
Sequence similarities
Belongs to the small GTPase superfamily. Rho family. -
Post-translational
modifications(Microbial infection) Glycosylated at Tyr-32 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rac and leads to actin disassembly. -
Cellular localization
Cytoplasm. Endomembrane system. Cell projection, lamellipodium. Cytoplasm, perinuclear region. Cell membrane. Cytoplasm, cytoskeleton. Membrane-associated when activated. Colocalizes with NRBP to endomembranes and at the cell periphery in lamellipodia. Colocalized with CIB1 in the perinuclear area and at the cell periphery. - Information by UniProt
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Database links
- Entrez Gene: 5881 Human
- Entrez Gene: 170758 Mouse
- Omim: 602050 Human
- SwissProt: P60763 Human
- SwissProt: P60764 Mouse
- Unigene: 45002 Human
- Unigene: 34008 Mouse
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Form
Rac3 has high sequence homology with other Rac proteins. Therefore some antibodies may show cross-reactivity. -
Alternative names
- OTTMUSP00000004488 antibody
- p21 Rac3 antibody
- p21-Rac3 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling RAC3 with Purified ab129062 at 1:100 dilution (2.64 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling RAC3 with Purified ab129062 at 1:100 dilution (2.64 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling RAC3 with Purified ab129062 at 1:100 dilution (2.64 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-RAC3 antibody [EPR6680] (ab129062) at 1/1000 dilution (Purified)
Lane 1 : Mouse brain lysates
Lane 2 : Rat brain lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaWe are unsure how to define the extra bands.
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Anti-RAC3 antibody [EPR6680] (ab129062) at 1/1000 dilution (Purified) + Human brain lysates at 20 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa -
All lanes : Anti-RAC3 antibody [EPR6680] (ab129062) at 1/1000 dilution (unpurified)
Lane 1 : 293T cell lysate
Lane 2 : Fetal brain lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa -
ab129062 (unpurified) at 1/50 dilution staining RAC3 in paraffin-embedded Human human breast carcinoma tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-RAC3 antibody [EPR6680] (ab129062) at 1/2000 dilution (unpurified)
Lane 1 : 293 cell extract
Lane 2 : 293 cell extract with Rac1 peptide
Lane 3 : 293 cell extract with Rac2 peptide
Lane 4 : 293 cell extract with Rac3 peptide
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution
Predicted band size: 21 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab129062 has been referenced in 6 publications.
- Kholmanskikh S et al. Activation of RhoC by regulatory ubiquitination is mediated by LNX1 and suppressed by LIS1. Sci Rep 12:16493 (2022). PubMed: 36192543
- Masi I et al. Endothelin-1 drives invadopodia and interaction with mesothelial cells through ILK. Cell Rep 34:108800 (2021). PubMed: 33657382
- Sun J et al. RAC1 plays an essential role in estrogen receptor alpha function in breast cancer cells. Oncogene 40:5950-5962 (2021). PubMed: 34373577
- Hampson E et al. P-Rex1 Controls Sphingosine 1-Phosphate Receptor Signalling, Morphology, and Cell-Cycle Progression in Neuronal Cells. Cells 10:N/A (2021). PubMed: 34572121
- Donnelly SK et al. Rac3 regulates breast cancer invasion and metastasis by controlling adhesion and matrix degradation. J Cell Biol 216:4331-4349 (2017). PubMed: 29061650
- Tang W et al. Suppressive action of miRNAs to ARP2/3 complex reduces cell migration and proliferation via RAC isoforms in Hirschsprung disease. J Cell Mol Med 20:1266-75 (2016). WB ; Human . PubMed: 26991540