Overview

  • Product name

    Anti-RAC3 antibody [EPR6680]
    See all RAC3 primary antibodies
  • Description

    Rabbit monoclonal [EPR6680] to RAC3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human RAC3 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • 293T, fetal brain, SH-SY5Y and JAR lysates. Human breast carcinoma tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as cell spreading and the formation of actin-based protusions including lamellipodia and membrane ruffles. Promotes cell adhesion and spreading on fibrinogen in a CIB1 and alpha-IIb/beta3 integrin-mediated manner.
    • Tissue specificity

      Highest levels in brain, also detected in heart, placenta and pancreas.
    • Sequence similarities

      Belongs to the small GTPase superfamily. Rho family.
    • Post-translational
      modifications

      (Microbial infection) Glycosylated at Tyr-32 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rac and leads to actin disassembly.
    • Cellular localization

      Cytoplasm. Endomembrane system. Cell projection, lamellipodium. Cytoplasm, perinuclear region. Cell membrane. Cytoplasm, cytoskeleton. Membrane-associated when activated. Colocalizes with NRBP to endomembranes and at the cell periphery in lamellipodia. Colocalized with CIB1 in the perinuclear area and at the cell periphery.
    • Information by UniProt
    • Database links

    • Form

      Rac3 has high sequence homology with other Rac proteins. Therefore some antibodies may show cross-reactivity.
    • Alternative names

      • OTTMUSP00000004488 antibody
      • p21 Rac3 antibody
      • p21-Rac3 antibody
      • Rac1B antibody
      • RAC3 antibody
      • RAC3_HUMAN antibody
      • RAS related C3 botulinum substrate 3 antibody
      • Ras related C3 botulinum toxin substrate 3 (rho family small GTP binding protein Rac3) antibody
      • Ras related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3) antibody
      • Ras-related C3 botulinum toxin substrate 3 antibody
      • Rho family small GTP binding protein Rac3 antibody
      • RP23-84C12.18 antibody
      see all

    Images

    • All lanes : Anti-RAC3 antibody [EPR6680] (ab129062) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Fetal brain lysate
      Lane 3 : SH-SY5Y cell lysate
      Lane 4 : JAR cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 21 kDa
      Observed band size: 21 kDa

    • ab129062 at 1/50 dilution staining RAC3 in paraffin-embedded Human human breast carcinoma tissue by immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • All lanes : Anti-RAC3 antibody [EPR6680] (ab129062) at 1/2000 dilution

      Lane 1 : 293 cell extract
      Lane 2 : 293 cell extract with Rac1 peptide
      Lane 3 : 293 cell extract with Rac2 peptide
      Lane 4 : 293 cell extract with Rac3 peptide

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

      Predicted band size: 21 kDa

    References

    This product has been referenced in:

    • Donnelly SK  et al. Rac3 regulates breast cancer invasion and metastasis by controlling adhesion and matrix degradation. J Cell Biol 216:4331-4349 (2017). Read more (PubMed: 29061650) »
    • Tang W  et al. Suppressive action of miRNAs to ARP2/3 complex reduces cell migration and proliferation via RAC isoforms in Hirschsprung disease. J Cell Mol Med 20:1266-75 (2016). WB ; Human . Read more (PubMed: 26991540) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab129062.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up