• Product name

    Anti-RACGAP1/MGCRACGAP antibody
    See all RACGAP1/MGCRACGAP primary antibodies
  • Description

    Goat polyclonal to RACGAP1/MGCRACGAP
  • Host species

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human RACGAP1/MGCRACGAP aa 620-632 (C terminal).


    (Peptide available as ab22898)

  • Positive control

    • Human testis lysate. K562 cells.
  • General notes

    Principal Names - RACGAP1/MGCRACGAP; Rac GTPase activating protein 1; ID-GAP; MgcRacGAP; GTPase activating protein. Official Gene Symbol - - RACGAP1/MGCRACGAP. GenBank Accession Number – NP_037409. LocusLink ID - 29127.

     This product was previously labelled as RACGAP1



  • Form

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab2270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 71 kDa).
IP Use at an assay dependent dilution.


  • Function

    Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.
  • Tissue specificity

    Highly expressed in testis, thymus and placenta. Expressed at lower levels in spleen and peripheral blood lymphocytes. In testis, expression is restricted to germ cells with the highest levels of expression found in spermatocytes. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase.
  • Sequence similarities

    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 Rho-GAP domain.
  • Domain

    The coiled coil region is indispensible for localization to the midbody during cytokinesis.
  • Post-translational

    Phosphorylated at multiple sites in the midbody during cytokinesis. Phosphorylation by AURKB on Ser-387 at the midbody is, at least in part, responsible for exerting its latent GAP activity towards RhoA. Phosphorylation on multiple serine residues by PLK1 enhances its association with ECT2 and is critical for cleavage furrow formation.
  • Cellular localization

    Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Cytoplasmic vesicle > secretory vesicle > acrosome. Cleavage furrow. Midbody. Colocalizes with RND2 in Golgi-derived proacrosomal vesicles and the acrosome (By similarity). During interphase, localized to the nucleus and cytoplasm along with microtubules, in anaphase, is redistributed to the central spindle and, in telophase and cytokinesis, to the midbody. Colocalizes with RHOA at the myosin contractile ring during cytokinesis. Colocalizes with ECT2 to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalizes with Cdc42 to spindle microtubules from prometaphase to telophase.
  • Information by UniProt
  • Database links

  • Alternative names

    • CYK4 antibody
    • GAP antibody
    • Gap1 antibody
    • GTPase activating protein antibody
    • HsCYK-4 antibody
    • ID GAP antibody
    • KIAA1478 antibody
    • Male germ cell RacGap antibody
    • MgcRacGAP antibody
    • Protein CYK4 homolg antibody
    • Protein CYK4 homolog antibody
    • Rac GTPase activating protein 1 antibody
    • Rac GTPase-activating protein 1 antibody
    • RACGAP 1 antibody
    • Racgap1 antibody
    • RGAP1_HUMAN antibody
    see all


  • All lanes :

    Lane 1 : K562 cell lysate (in RIPA buffer)
    Lane 2 : Jurkat cell lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 71 kDa

    Primary incubation was 1 hour. Detected by chemiluminescence.


  • ab2270 (1µg/ml) staining Human Testis by IHC-P. Microwaved antigen retrieval with Tris/EDTA buffer (pH9), HRP-staining.
  • Anti-RACGAP1/MGCRACGAP antibody (ab2270) at 0.2 µg/ml + Human Testis lysate (RIPA buffer) at 30 µg

    Predicted band size: 71 kDa

    ab2270 staining (0.2µg/ml) of Human Testis lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour.  Detected by western blot using chemiluminescence.

    Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

  • Anti-RACGAP1/MGCRACGAP antibody (ab2270) at 1 µg/ml + K562 cell lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 71 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?


This product has been referenced in:

  • Khalid M  et al. Gene Regulation by Antitumor miR-204-5p in Pancreatic Ductal Adenocarcinoma: The Clinical Significance of Direct RACGAP1 Regulation. Cancers (Basel) 11:N/A (2019). Read more (PubMed: 30866526) »
  • Barbiero I  et al. CDKL5 localizes at the centrosome and midbody and is required for faithful cell division. Sci Rep 7:6228 (2017). Read more (PubMed: 28740074) »
See all 20 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence
Human Cell (HT1080)
Yes - 0.5% Triton X100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jan 21 2015

Western blot
Human Cell lysate - whole cell (HT1080)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
20 µg
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Jan 13 2015

Western blot
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 04 2013

Immunocytochemistry/ Immunofluorescence
Human Cell (HeLa)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 27°C

Abcam user community

Verified customer

Submitted Dec 16 2008


I used Lysis buffer consisting of the following components: 1% Triton X-100, 0.5% NP-40, 150mM NaCl, 10mM Tris-HCL (pH 7.4), 1x protease inhibitor, 1 mM EDTA to lyse the cell pellets (liver cell lines harvested using 0.25% trypsin with EDTA) at 4oC for 40 to 60 min. Then the lysate were spun for 13K rpm at 4oC for 10 min. The supernatant was then transfered to a new tube and 1 ul was used for quantitation using Bio-rad Protein assay. I mix the protein( 40 ug each sample) with 5x sample buffer (with final concentration containing 0.3M Tris-HCL pH 6.8, 10% SDS, 30% Glycerol, 10% Mercaptoethanol, ~4% Bromophenol Blue) and ddH2 O to make up the final volume of 50 ul so as to give a final 1x sample buffer. The mixture were boiled (100oC) for 5 min and quick spun before loading onto SDS-PAGE gels (10% resolving and 4% stacking gels). After transfering onto Nylon membrane, the membrane was blocked using skim milk powder for about 40 to 60 min before being probed by 0.4ug/ml RACGAP1 antibody (ab2270) at 4oC for overnight. The next day , the blot was washed with 3 times 1xPBS containing 0.05% Tween-20 before being probed by secondary Ab (1:10k diluted rabbit anti-goat HRP conjugated Ab). The wash was repeated for 3 times before staining with 1: 1 mix of Immobilon Western HRP-substrate peroxide and HRP-substrate Luminol (Millipore) for 5 min at room temp. May I know the detail protocol on how to prepare the lysate (HumanTestis lysate) in the ab2270 datasheet and is it very different from what I performed (e.g: lysis, gel condition etc). I wonder the extra band (lower MW - between 38.3 kDa and 55.9 kDa) is the phophosrylated form of this protein?? Hear from you urgently.

Read More

Thank you for your e-mail. The lysate used was Human Testis lysate lysed in RIPA buffer, the recipe for that buffer is as detailed below: 50mM Tris HCl pH 8 150 mM NaCl 1% NP-40 0.5% sodium Deoxycholate 0.1% SDS protease inhibitor cocktail (Roche or Sigma) I do not think the lower band can be the phosphorylated protein as phosphorylated proteins have relatively the same MW as non phosphorylated (if not they are a little higher but this is often not detectable by western blotting) and I think the lower band is more likely to be a degradation product (if your protease inhibitors are not efficient enough (not enough or too old)) or a fragment from post translational cleavage. I would recommend to run the positive control to see if you observe that band, and also to run a no primary control and to try blocking with BSA 5% 1 hour in case it is the blocking agent creating this problem. If you can also try overnight blocking in milk this may also be a good modification to try (if you have plenty of samples). Please let me know how you get on with those modifications and do not hesitate to contact me if you need further assistance,

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Thank you for your enquiry. This antibody has been tested on Human Testis lysate, therefore we would strongly suggest using this type of lysate as positive control along with the samples. Should your customer still have difficulty with this product, then please provide some more details of his assay.

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