Recombinant
RabMAb

Recombinant Anti-RACGAP1/MGCRACGAP antibody [EPR9018] (ab134972)

Overview

  • Product name

    Anti-RACGAP1/MGCRACGAP antibody [EPR9018]
    See all RACGAP1/MGCRACGAP primary antibodies
  • Description

    Rabbit monoclonal [EPR9018] to RACGAP1/MGCRACGAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human RACGAP1/MGCRACGAP aa 50-150. The exact sequence is proprietary.

  • Positive control

    • A431, Raji, Jurkat, and JAR cell lysates; Human testis tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as RACGAP1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.
    • Tissue specificity

      Highly expressed in testis, thymus and placenta. Expressed at lower levels in spleen and peripheral blood lymphocytes. In testis, expression is restricted to germ cells with the highest levels of expression found in spermatocytes. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase.
    • Sequence similarities

      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 Rho-GAP domain.
    • Domain

      The coiled coil region is indispensible for localization to the midbody during cytokinesis.
    • Post-translational
      modifications

      Phosphorylated at multiple sites in the midbody during cytokinesis. Phosphorylation by AURKB on Ser-387 at the midbody is, at least in part, responsible for exerting its latent GAP activity towards RhoA. Phosphorylation on multiple serine residues by PLK1 enhances its association with ECT2 and is critical for cleavage furrow formation.
    • Cellular localization

      Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Cytoplasmic vesicle > secretory vesicle > acrosome. Cleavage furrow. Midbody. Colocalizes with RND2 in Golgi-derived proacrosomal vesicles and the acrosome (By similarity). During interphase, localized to the nucleus and cytoplasm along with microtubules, in anaphase, is redistributed to the central spindle and, in telophase and cytokinesis, to the midbody. Colocalizes with RHOA at the myosin contractile ring during cytokinesis. Colocalizes with ECT2 to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalizes with Cdc42 to spindle microtubules from prometaphase to telophase.
    • Information by UniProt
    • Database links

    • Alternative names

      • CYK4 antibody
      • GAP antibody
      • Gap1 antibody
      • GTPase activating protein antibody
      • HsCYK-4 antibody
      • ID GAP antibody
      • KIAA1478 antibody
      • Male germ cell RacGap antibody
      • MgcRacGAP antibody
      • Protein CYK4 homolg antibody
      • Protein CYK4 homolog antibody
      • Rac GTPase activating protein 1 antibody
      • Rac GTPase-activating protein 1 antibody
      • RACGAP 1 antibody
      • Racgap1 antibody
      • RGAP1_HUMAN antibody
      see all

    Images

    • All lanes : Anti-RACGAP1/MGCRACGAP antibody [EPR9018] (ab134972) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : JAR cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 71 kDa
      Observed band size: 71 kDa

    • Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling RACGAP1/MGCRACGAP with ab134972 at 1/50 dilution.

    References

    ab134972 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134972.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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