Overview

  • Product name

    Anti-RACGAP1/MGCRACGAP (phospho S387) antibody
    See all RACGAP1/MGCRACGAP primary antibodies
  • Description

    Rabbit polyclonal to RACGAP1/MGCRACGAP (phospho S387)
  • Host species

    Rabbit
  • Specificity

    ab193220 detects endogenous level of RACGAP1/MGCRACGAP only when phosphorylated at Serine 387.

  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Monkey
  • Immunogen

    Synthetic peptide within Human RACGAP1/MGCRACGAP (phospho S387) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary. NCBI Protein#: NP_001119575.1
    Database link: Q9H0H5

  • Positive control

    • COS7 tissue extracts.
  • General notes

     This product was previously labelled as RACGAP1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride

    PBS is without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab193220 was purified by affinity-chromatography using an-epitope specific phosphopeptide. Non-phosphopeptide specific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab193220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 71 kDa.

Target

  • Function

    Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.
  • Tissue specificity

    Highly expressed in testis, thymus and placenta. Expressed at lower levels in spleen and peripheral blood lymphocytes. In testis, expression is restricted to germ cells with the highest levels of expression found in spermatocytes. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase.
  • Sequence similarities

    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 Rho-GAP domain.
  • Domain

    The coiled coil region is indispensible for localization to the midbody during cytokinesis.
  • Post-translational
    modifications

    Phosphorylated at multiple sites in the midbody during cytokinesis. Phosphorylation by AURKB on Ser-387 at the midbody is, at least in part, responsible for exerting its latent GAP activity towards RhoA. Phosphorylation on multiple serine residues by PLK1 enhances its association with ECT2 and is critical for cleavage furrow formation.
  • Cellular localization

    Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Cytoplasmic vesicle > secretory vesicle > acrosome. Cleavage furrow. Midbody. Colocalizes with RND2 in Golgi-derived proacrosomal vesicles and the acrosome (By similarity). During interphase, localized to the nucleus and cytoplasm along with microtubules, in anaphase, is redistributed to the central spindle and, in telophase and cytokinesis, to the midbody. Colocalizes with RHOA at the myosin contractile ring during cytokinesis. Colocalizes with ECT2 to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalizes with Cdc42 to spindle microtubules from prometaphase to telophase.
  • Information by UniProt
  • Database links

  • Alternative names

    • CYK4 antibody
    • GAP antibody
    • Gap1 antibody
    • GTPase activating protein antibody
    • HsCYK-4 antibody
    • ID GAP antibody
    • KIAA1478 antibody
    • Male germ cell RacGap antibody
    • MgcRacGAP antibody
    • Protein CYK4 homolg antibody
    • Protein CYK4 homolog antibody
    • Rac GTPase activating protein 1 antibody
    • Rac GTPase-activating protein 1 antibody
    • RACGAP 1 antibody
    • Racgap1 antibody
    • RGAP1_HUMAN antibody
    see all

Images

  • All lanes : Anti-RACGAP1/MGCRACGAP (phospho S387) antibody (ab193220) at 1/500 dilution

    Lane 1 : COS7 tissue extracts
    Lane 2 : COS7 tissue extracts with antigen-specific peptide

    Predicted band size: 71 kDa

References

ab193220 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab193220.
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