Key features and details
- Goat polyclonal to RACK1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-RACK1 antibody
See all RACK1 primary antibodies
DescriptionGoat polyclonal to RACK1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Horse, Chicken, Hamster, Cow, Dog, Pig, Monkey, Gorilla
- Human Small Intestine tissue; Human Lymph Node lysate; Mouse and Rat Spleen lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab166796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 35 kDa).|
|IHC-P||Use a concentration of 3.75 µg/ml.|
FunctionSeems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation.
Sequence similaritiesBelongs to the WD repeat G protein beta family.
Contains 7 WD repeats.
DomainThe WD repeats domain 5 mediates interaction with TRIM63.
Cellular localizationCell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
- Information by UniProt
- Cell proliferation-inducing gene 21 protein antibody
- GBLP_HUMAN antibody
- Gnb2-rs1 antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue labeling RACK1 with ab166796 at 3.75 µg/ml.
Anti-RACK1 antibody (ab166796) at 0.1 µg/ml + Human Lymph Node lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
Primary incubation: 1 hour.
ab166796 has not yet been referenced specifically in any publications.