Product nameAnti-RACK1 antibody
See all RACK1 primary antibodies
DescriptionRabbit polyclonal to RACK1
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Pig, Xenopus laevis, Cynomolgus monkey
- Paraformaldehyde-fixed HeLa cells, HEK-293T cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
Purification notesThe antiserum was fractionated and further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
Our Abpromise guarantee covers the use of ab62735 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 35 kDa).|
|IP||Use at an assay dependent dilution. Use 5-10 µL.|
|ICC/IF||1/100 - 1/200.|
FunctionSeems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation.
Sequence similaritiesBelongs to the WD repeat G protein beta family.
Contains 7 WD repeats.
DomainThe WD repeats domain 5 mediates interaction with TRIM63.
Cellular localizationCell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
- Information by UniProt
- Cell proliferation-inducing gene 21 protein antibody
- GBLP_HUMAN antibody
- Gnb2-rs1 antibody
All lanes : Anti-RACK1 antibody (ab62735) at 1/500 dilution
Lane 1 : Extract from hek-293T cell line
Lane 2 : Extract from HeLa cell line
Lane 3 : Extract from MCF7 cell line
Lane 4 : Extract from U2OS cell line
Lane 5 : Extract from Jurkat cell line
Lane 6 : Extract from RAT1 cell line
Lane 7 : Extract from PC12 cell line
Lane 8 : Extract from NIH3T3 cell line
All lanes : Goat Anti-Rabbit IgG
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Chen Y et al. O-GlcNAcylated c-Jun antagonizes ferroptosis via inhibiting GSH synthesis in liver cancer. Cell Signal 63:109384 (2019). Read more (PubMed: 31394193) »
- Feng J et al. RACK1 silencing attenuates renal fibrosis by inhibiting TGF-ß signaling. Int J Mol Med 40:1965-1970 (2017). Read more (PubMed: 29039466) »