Product nameAnti-RACK1 antibody [EPR7388]
See all RACK1 primary antibodies
DescriptionRabbit monoclonal [EPR7388] to RACK1
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues in Human RACK1 (UniProt ID: P63244).
- MCF7, SH-SY5Y, HeLa, PC12, and C6 cell lysates, Human cervical carcinoma tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab129084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 35 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionSeems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation.
Sequence similaritiesBelongs to the WD repeat G protein beta family.
Contains 7 WD repeats.
DomainThe WD repeats domain 5 mediates interaction with TRIM63.
Cellular localizationCell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
- Information by UniProt
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All lanes : Anti-RACK1 antibody [EPR7388] (ab129084) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : PC12 cell lysate
Lane 5 : C6 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 35 kDa
ab129084, at a dilution of 1/50, staining RACK1 in paraffin-embedded Human cervical carcinoma tissue by Immunohistochemistry.
This product has been referenced in:
- Li XY et al. RACK1 Acts as a Potential Tumor Promoter in Colorectal Cancer. Gastroenterol Res Pract 2019:5625026 (2019). Read more (PubMed: 30962803) »
- Corcoran KA et al. Regulation of fear extinction versus other affective behaviors by discrete cortical scaffolding complexes associated with NR2B and PKA signaling. Transl Psychiatry 5:e657 (2015). Read more (PubMed: 26460481) »