Recombinant
RabMAb

Recombinant Anti-RACK1 antibody [EPR7388] (ab129084)

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Overview

  • Product name

    Anti-RACK1 antibody [EPR7388]
    See all RACK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7388] to RACK1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human RACK1 (UniProt ID: P63244).

  • Positive control

    • WB: HeLa, HepG2, SH-SY5Y, MCF7, C6, PC-12, Mouse brain, and NIH/3T3 lysates. IHC-P: human colon carcinoma, and cervical carcinoma, mouse cerebrum, and rat cerebrum tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab129084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 35 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/50 -1/100
  • Application notes
    Is unsuitable for ICC or IP.

    • Target

    • Function

      Seems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation.

    • Sequence similarities

      Belongs to the WD repeat G protein beta family.
      Contains 7 WD repeats.

    • Domain

      The WD repeats domain 5 mediates interaction with TRIM63.

    • Cellular localization

      Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Cell proliferation-inducing gene 21 protein antibody
      • GBLP_HUMAN antibody
      • Gnb2-rs1 antibody
      • Gnb2l1 antibody
      • Guanine nucleotide binding protein (G protein) beta polypeptide 2 like 1 antibody
      • Guanine nucleotide binding protein beta polypeptide 2 like 1 antibody
      • Guanine nucleotide binding protein beta subunit 2 like 1 antibody
      • Guanine nucleotide binding protein beta subunit like protein 12.3 antibody
      • Guanine nucleotide binding protein subunit beta 2 like 1 antibody
      • Guanine nucleotide binding protein subunit beta like protein 12.3 antibody
      • Guanine nucleotide-binding protein subunit beta-2-like 1 antibody
      • Guanine nucleotide-binding protein subunit beta-like protein 12.3 antibody
      • H12.3 antibody
      • HLC-7 antibody
      • Human lung cancer oncogene 7 protein antibody
      • lung cancer oncogene 7 antibody
      • OTTHUMP00000223704 antibody
      • OTTHUMP00000223870 antibody
      • OTTHUMP00000223891 antibody
      • OTTHUMP00000223893 antibody
      • OTTHUMP00000223900 antibody
      • OTTHUMP00000223902 antibody
      • OTTHUMP00000223930 antibody
      • OTTHUMP00000223931 antibody
      • PIG21 antibody
      • Proliferation inducing gene 21 antibody
      • Protein homologous to chicken B complex protein guanine nucleotide binding antibody
      • RACK1 antibody
      • Receptor for activated C kinase 1 antibody
      • Receptor for activated C kinase antibody
      • Receptor of activated protein kinase C 1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (ab129084)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (ab129084)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling RACK1 with Purified ab129084 at 1:1000 dilution (0.201 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (ab129084)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (ab129084)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling RACK1 with Purified ab129084 at 1:1000 dilution (0.201 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (ab129084)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (ab129084)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue sections labeling RACK1 with Purified ab129084 at 1:1000 dilution (0.201 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Western blot - Anti-RACK1 antibody [EPR7388] (ab129084)
      Western blot - Anti-RACK1 antibody [EPR7388] (ab129084)
      All lanes : Anti-RACK1 antibody [EPR7388] (ab129084) at 1/1000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
      Lane 3 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
      Lane 4 : C6 (Rat glial tumor glial cell) whole cell lysates
      Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
      Lane 6 : Mouse brain lysates
      Lane 7 : Mouse spleen lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 35 kDa
      Observed band size: 32 kDa
      why is the actual band size different from the predicted?

    • Western blot - Anti-RACK1 antibody [EPR7388] (ab129084)
      Western blot - Anti-RACK1 antibody [EPR7388] (ab129084)
      All lanes : Anti-RACK1 antibody [EPR7388] (ab129084) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : PC12 cell lysate
      Lane 5 : C6 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 35 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (ab129084)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (ab129084)

      ab129084, at a dilution of 1/50, staining RACK1 in paraffin-embedded Human cervical carcinoma tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Li XY  et al. RACK1 Acts as a Potential Tumor Promoter in Colorectal Cancer. Gastroenterol Res Pract 2019:5625026 (2019). Read more (PubMed: 30962803) »
    • Corcoran KA  et al. Regulation of fear extinction versus other affective behaviors by discrete cortical scaffolding complexes associated with NR2B and PKA signaling. Transl Psychiatry 5:e657 (2015). Read more (PubMed: 26460481) »
    See all 2 Publications for this product

    Publishing research using ab129084? Please let us know so that we can cite the reference in this datasheet.

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