Recombinant
RabMAb

Recombinant Anti-RACK1 antibody [EPR7388] (ab129084)

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Overview

  • Product name

    Anti-RACK1 antibody [EPR7388]
    See all RACK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7388] to RACK1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human RACK1 (UniProt ID: P63244).

  • Positive control

    • MCF7, SH-SY5Y, HeLa, PC12, and C6 cell lysates, Human cervical carcinoma tissue

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 35 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC or IP.

    • Target

    • Function

      Seems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation.

    • Sequence similarities

      Belongs to the WD repeat G protein beta family.
      Contains 7 WD repeats.

    • Domain

      The WD repeats domain 5 mediates interaction with TRIM63.

    • Cellular localization

      Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Cell proliferation-inducing gene 21 protein antibody
      • GBLP_HUMAN antibody
      • Gnb2-rs1 antibody
      • Gnb2l1 antibody
      • Guanine nucleotide binding protein (G protein) beta polypeptide 2 like 1 antibody
      • Guanine nucleotide binding protein beta polypeptide 2 like 1 antibody
      • Guanine nucleotide binding protein beta subunit 2 like 1 antibody
      • Guanine nucleotide binding protein beta subunit like protein 12.3 antibody
      • Guanine nucleotide binding protein subunit beta 2 like 1 antibody
      • Guanine nucleotide binding protein subunit beta like protein 12.3 antibody
      • Guanine nucleotide-binding protein subunit beta-2-like 1 antibody
      • Guanine nucleotide-binding protein subunit beta-like protein 12.3 antibody
      • H12.3 antibody
      • HLC-7 antibody
      • Human lung cancer oncogene 7 protein antibody
      • lung cancer oncogene 7 antibody
      • OTTHUMP00000223704 antibody
      • OTTHUMP00000223870 antibody
      • OTTHUMP00000223891 antibody
      • OTTHUMP00000223893 antibody
      • OTTHUMP00000223900 antibody
      • OTTHUMP00000223902 antibody
      • OTTHUMP00000223930 antibody
      • OTTHUMP00000223931 antibody
      • PIG21 antibody
      • Proliferation inducing gene 21 antibody
      • Protein homologous to chicken B complex protein guanine nucleotide binding antibody
      • RACK1 antibody
      • Receptor for activated C kinase 1 antibody
      • Receptor for activated C kinase antibody
      • Receptor of activated protein kinase C 1 antibody
      see all

    Images

    • Western blot - Anti-RACK1 antibody [EPR7388] (ab129084)
      Western blot - Anti-RACK1 antibody [EPR7388] (ab129084)
      All lanes : Anti-RACK1 antibody [EPR7388] (ab129084) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : PC12 cell lysate
      Lane 5 : C6 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 35 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (ab129084)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (ab129084)
      ab129084, at a dilution of 1/50, staining RACK1 in paraffin-embedded Human cervical carcinoma tissue by Immunohistochemistry.

    References

    This product has been referenced in:

    • Li XY  et al. RACK1 Acts as a Potential Tumor Promoter in Colorectal Cancer. Gastroenterol Res Pract 2019:5625026 (2019). Read more (PubMed: 30962803) »
    • Corcoran KA  et al. Regulation of fear extinction versus other affective behaviors by discrete cortical scaffolding complexes associated with NR2B and PKA signaling. Transl Psychiatry 5:e657 (2015). Read more (PubMed: 26460481) »
    See all 2 Publications for this product

    Publishing research using ab129084? Please let us know so that we can cite the reference in this datasheet.

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