Anti-Rad1 antibody (ab168397)
Key features and details
- Mouse polyclonal to Rad1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Rad1 antibody
See all Rad1 primary antibodies -
Description
Mouse polyclonal to Rad1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Chinese hamster, Orangutan -
Immunogen
Recombinant full length protein, corresponding to amino acids 1-282 of Human Rad1 (NP_002844).
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Positive control
- Rad1 transfected 293T cell line lysate, HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168397 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity. -
Tissue specificity
Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level). -
Sequence similarities
Belongs to the rad1 family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5810 Human
- Omim: 603153 Human
- SwissProt: O60671 Human
- Unigene: 38114 Human
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Alternative names
- Cell cycle checkpoint protein Hrad1 antibody
- Cell cycle checkpoint protein Rad 1 A / B antibody
- Cell cycle checkpoint protein RAD1 antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab168397 has not yet been referenced specifically in any publications.