Anti-Rad1 antibody (ab168397)
Key features and details
- Mouse polyclonal to Rad1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Rad1 antibody
See all Rad1 primary antibodies -
Description
Mouse polyclonal to Rad1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Chinese hamster, Orangutan
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Immunogen
Recombinant full length protein, corresponding to amino acids 1-282 of Human Rad1 (NP_002844).
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Positive control
- Rad1 transfected 293T cell line lysate, HeLa cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab168397 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
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Function
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity. -
Tissue specificity
Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level). -
Sequence similarities
Belongs to the rad1 family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5810 Human
- Omim: 603153 Human
- SwissProt: O60671 Human
- Unigene: 38114 Human
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Alternative names
- Cell cycle checkpoint protein Hrad1 antibody
- Cell cycle checkpoint protein Rad 1 A / B antibody
- Cell cycle checkpoint protein RAD1 antibody
see all
Images
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Western blot - Anti-Rad1 antibody (ab168397)All lanes : Anti-Rad1 antibody (ab168397) at 1 µg/ml
Lane 1 : Rad1 transfected 293T cell line lysate
Lane 2 : Non-transfected 293T cell line lysate
Predicted band size: 32 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Rad1 antibody (ab168397)Immunofluorescent analysis of HeLa cells labeling Rad1 with ab168397 at 10 µg/ml.
Protocols
Datasheets and documents
References (0)
ab168397 has not yet been referenced specifically in any publications.
