Anti-Rad1 antibody (ab168397)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Rad1 antibody
See all Rad1 primary antibodies -
Description
Mouse polyclonal to Rad1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Chinese hamster, Orangutan
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Immunogen
Recombinant full length protein, corresponding to amino acids 1-282 of Human Rad1 (NP_002844).
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Positive control
- Rad1 transfected 293T cell line lysate, HeLa cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.20
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab168397 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
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Function
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity. -
Tissue specificity
Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level). -
Sequence similarities
Belongs to the rad1 family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5810 Human
- Omim: 603153 Human
- SwissProt: O60671 Human
- Unigene: 38114 Human
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Alternative names
- Cell cycle checkpoint protein Hrad1 antibody
- Cell cycle checkpoint protein Rad 1 A / B antibody
- Cell cycle checkpoint protein RAD1 antibody
see all
Images
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Western blot - Anti-Rad1 antibody (ab168397)All lanes : Anti-Rad1 antibody (ab168397) at 1 µg/ml
Lane 1 : Rad1 transfected 293T cell line lysate
Lane 2 : Non-transfected 293T cell line lysate
Predicted band size: 32 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Rad1 antibody (ab168397)Immunofluorescent analysis of HeLa cells labeling Rad1 with ab168397 at 10 µg/ml.
Datasheets and documents
References
ab168397 has not yet been referenced specifically in any publications.
