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    rad1-antibody-ab168397.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair DNA Damage Response DNA Damage Recognition
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Anti-Rad1 antibody (ab168397)

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Western blot - Anti-Rad1 antibody (ab168397)
  • Immunocytochemistry/ Immunofluorescence - Anti-Rad1 antibody (ab168397)

Key features and details

  • Mouse polyclonal to Rad1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Rad1 antibody
    See all Rad1 primary antibodies
  • Description

    Mouse polyclonal to Rad1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Chinese hamster, Orangutan
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-282 of Human Rad1 (NP_002844).

  • Positive control

    • Rad1 transfected 293T cell line lysate, HeLa cells
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.4
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • DNA Damage Recognition

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG - Isotype Control (ab37355)
  • Recombinant Protein

    • Recombinant Human Rad1 protein (ab97946)
  • Related Products

    • Recombinant Human Rad1 protein (ab97946)

Applications

Our Abpromise guarantee covers the use of ab168397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.
  • Tissue specificity

    Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level).
  • Sequence similarities

    Belongs to the rad1 family.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession O60671 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5810 Human
    • Omim: 603153 Human
    • SwissProt: O60671 Human
    • Unigene: 38114 Human
    • Alternative names

      • Cell cycle checkpoint protein Hrad1 antibody
      • Cell cycle checkpoint protein Rad 1 A / B antibody
      • Cell cycle checkpoint protein RAD1 antibody
      • Checkpoint control protein HRAD1 antibody
      • Checkpoint control protein RAD1 antibody
      • DNA repair exonuclease antibody
      • DNA repair exonuclease rad1 antibody
      • DNA repair exonuclease rad1 homolog antibody
      • DNA repair exonuclease REC1 antibody
      • DNA repair protein RAD1 antibody
      • EC 3.1.11.2 antibody
      • Exonuclease homolog RAD1 antibody
      • GTP-binding protein RAD antibody
      • hRAD 1 antibody
      • hRAD1 antibody
      • MGC77779 antibody
      • RAD 1 antibody
      • RAD antibody
      • RAD1 antibody
      • RAD1 homolog (S. pombe) antibody
      • RAD1 homolog antibody
      • Rad1 like DNA damage checkpoint antibody
      • Rad1 like DNA damage checkpoint protein antibody
      • RAD1, S. pombe, homolog of antibody
      • Rad1-like DNA damage checkpoint protein antibody
      • RAD1_HUMAN antibody
      • Ras associated with diabetes antibody
      • REC 1 antibody
      • REC1 antibody
      • RRAD antibody
      see all

    Images

    • Western blot - Anti-Rad1 antibody (ab168397)
      Western blot - Anti-Rad1 antibody (ab168397)
      All lanes : Anti-Rad1 antibody (ab168397) at 1 µg/ml

      Lane 1 : Rad1 transfected 293T cell line lysate
      Lane 2 : Non-transfected 293T cell line lysate

      Predicted band size: 32 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Rad1 antibody (ab168397)
      Immunocytochemistry/ Immunofluorescence - Anti-Rad1 antibody (ab168397)
      Immunofluorescent analysis of HeLa cells labeling Rad1 with ab168397 at 10 µg/ml.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab168397? Please let us know so that we can cite the reference in this datasheet.

    ab168397 has not yet been referenced specifically in any publications.

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