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I ordered an anti-pRad17 antibody (Ab3620) recently and tested on my cell lysates for western blot.
I started with cells with and without radiation and expected an enhanced pRad17 activity in cells with radiation.
However, I didn't get any signal from my blot.
I'm wondering if you can help me troubleshooting with my western blot experiment?
Asked on Jan 23 2012
I am sorry to hear that you have been experiencing problems using this product in the application that you wish.
In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application used. Often it is possible to make suggestions that may help resolve problems experienced using a particular product.
As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.
All our customer feedback, including complaints are monitored weekly by our in house technical support team. If a product is at fault the technical support team will consider removing the product from our catalogue in order to avoid future customer inconvenience.
Please could you provide some further details of the protocol used and complete the following form (attached as a word document). It would be much appreciated if you could attach an image to the response.
Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.
Answered on Jan 23 2012