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Thanks for your kind reply.
I used human glioma cell line U251 in my study, and I have seen paper reported glioma cells have this pRad activity.
I stripped and reblotted with Actin and got pretty good signal, which i guess means the transfer was good.
I think what you said means using BSA for blocking can only reduce the background noise.
Since I didn't even get any specific binding, I guess try again with BSA may not have signficant effect for my experiment.
Asked on Feb 22 2012
Well that's true. BSA reduces the background only, it does not have any effect on ab specificity.
Anyway to proceed further in this case, I would like to ask if you are happy in trying a different lot or a different antibody. Alternatively I can also provide you full refund or credit note, which you can use for future purchases. Let me know the option you would like to go for.
Many thanks for your cooperation in this case, I highly appreciate that.
Answered on Feb 22 2012