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I am interested in your human rad 17 antibodies and was concerned that your goat polyclonal recognises a 90 kD protein, whereas your rabbit polyclonal recognises a 69kD. The phospho specific ( and therefore I assume slower running) comes in at 85-88kD. Is there a reason that your polyclonal goat recognises larger protein?
Asked on May 18 2003
To our knowledge, these three antibodies have not been used in parallel, which would be required to determine if there are truely differences in the apparant molecular weight of the protein recognized by each antibody. The differences in the reported apparant molecular weights of the proteins detected by the 3 antibodies is likely owing to differences in molecular weight markers that where used, position of the sample relative to the positon of the molecular weight markers in the gel, and size and composition of the resolving gels. The difference in relative mobility of the protein recognized by the goat anti-Rad17 and the phospho specific antibody is certainly within experimental error.
Answered on May 20 2003