RecombinantRabMAb

Recombinant Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012)

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Western blot - Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012)
  • Immunocytochemistry/ Immunofluorescence - Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012)
  • Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3145] to Rad17 (phospho S656)
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Rad17 (phospho S656) antibody [EPR3145]
    See all Rad17 primary antibodies

  • Description

    Rabbit monoclonal [EPR3145] to Rad17 (phospho S656)

  • Host species

    Rabbit

  • Specificity

    ab76012 only detects Rad17 phosphoryated on Serine 656.

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    A phospho-specific peptide corresponding to residues surrounding Serine 656 of human Rad17.

  • Positive control

    • HeLa cell lysate treated with Calyculin A. Human breast carcinoma. HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR3145

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
IHC-P
Notes
  • Application notes
    ICC/IF: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.
    WB: 1/5000 - 1/10000. Predicted molecular weight: 77 kDa.

    Is unsuitable for Flow Cyt or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.

    • Tissue specificity

      Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors).

    • Sequence similarities

      Belongs to the rad17/RAD24 family.

    • Post-translational
      modifications

      Phosphorylated. Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4.

    • Cellular localization

      Nucleus. Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.
    • Information by UniProt

    • Database links

      • Alternative names

        • CCYC antibody
        • Cell cycle checkpoint protein antibody
        • Cell cycle checkpoint protein RAD17 antibody
        • FLJ41520 antibody
        • HRAD 17 antibody
        • hRad17 antibody
        • R24L antibody
        • Rad 17 antibody
        • Rad 24 antibody
        • RAD1 (S. pombe) homolog antibody
        • RAD1 homolog antibody
        • RAD17 antibody
        • RAD17 homolog (S. pombe) antibody
        • RAD17 homolog antibody
        • Rad17 like protein antibody
        • RAD17, S. pombe, homolog of antibody
        • RAD17_HUMAN antibody
        • RAD17Sp antibody
        • Rad24 antibody
        • Rad24, mouse, homolog of antibody
        • Rad24, S. cerevisiae, homolog of antibody
        • RF C activator 1 homolog antibody
        • RF C/activator 1 homolog antibody
        • RF-C/activator 1 homolog antibody
        see all

      Images

      • Western blot - Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012)
        Western blot - Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012)
        All lanes : Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012) at 1/10000 dilution

        Lane 1 : HeLa cell lysate, untreated
        Lane 2 : HeLa cell lysate treated with
        Calyculin A

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 77 kDa
        Observed band size: 80 kDa why is the actual band size different from the predicted?

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012)

        ab76012, at 1/100-1/250 dilution, staining Rad17 in human breast carcinoma by immunohistochemistry using paraffin-embedded tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunocytochemistry/ Immunofluorescence - Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012)
        Immunocytochemistry/ Immunofluorescence - Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012)
        ab76012, at 1/100-1/250 dilution, staining Rad17 in HeLa cells by immunofluorescence.
      • Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012)
        Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012)

      References (0)

      Publishing research using ab76012? Please let us know so that we can cite the reference in this datasheet.

      ab76012 has not yet been referenced specifically in any publications.

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