Recombinant
Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Rad17 (phospho S656) antibody [EPR3145]
See all Rad17 primary antibodies -
DescriptionRabbit monoclonal [EPR3145] to Rad17 (phospho S656)
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Host speciesRabbit
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Specificityab76012 only detects Rad17 phosphoryated on Serine 656.
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Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivityReacts with: Human
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Immunogen
A phospho-specific peptide corresponding to residues surrounding Serine 656 of human Rad17.
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Positive control
- HeLa cell lysate treated with Calyculin A. Human breast carcinoma. HeLa cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR3145
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IsotypeIgG
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Research areas
Applications
Our Abpromise guarantee covers the use of ab76012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | ||
| WB | ||
| IHC-P |
Application notes
ICC/IF: 1/100 - 1/250.
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.
WB: 1/5000 - 1/10000. Predicted molecular weight: 77 kDa.
Is unsuitable for Flow Cyt or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.
WB: 1/5000 - 1/10000. Predicted molecular weight: 77 kDa.
Is unsuitable for Flow Cyt or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionEssential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
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Tissue specificityOverexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors).
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Sequence similaritiesBelongs to the rad17/RAD24 family.
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Post-translational
modificationsPhosphorylated. Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4. -
Cellular localizationNucleus. Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.
- Information by UniProt
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Database links
- Entrez Gene: 5884 Human
- Omim: 603139 Human
- SwissProt: O75943 Human
- Unigene: 16184 Human
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Alternative names
- CCYC antibody
- Cell cycle checkpoint protein antibody
- Cell cycle checkpoint protein RAD17 antibody
see all
Images
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![Western blot - Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012)](https://www.abcam.com/ps/products/76/ab76012/Images/ab76012-1.jpg)
Western blot - Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012)All lanes : Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012) at 1/10000 dilution
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate treated with
Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 77 kDa
Observed band size: 80 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012)](https://www.abcam.com/ps/products/76/ab76012/Images/ab76012-2.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012)ab76012, at 1/100-1/250 dilution, staining Rad17 in human breast carcinoma by immunohistochemistry using paraffin-embedded tissue. -
![Immunocytochemistry/ Immunofluorescence - Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012)](https://www.abcam.com/ps/products/76/ab76012/Images/ab76012-3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012)ab76012, at 1/100-1/250 dilution, staining Rad17 in HeLa cells by immunofluorescence.
Protocols
References
ab76012 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"