Product nameAnti-RAD18 antibody
See all RAD18 primary antibodies
DescriptionRabbit polyclonal to RAD18
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human RAD18 aa 1-495.
Database link: Q9NS91
- Extracts from K562, MCF7, Jurkat, 293T and mouse liver
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab186835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/500 - 1/2000. Predicted molecular weight: 56 kDa.|
|IHC-P||1/50 - 1/200.|
FunctionE3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the RAD18 family.
Contains 1 RING-type zinc finger.
Contains 1 SAP domain.
Contains 1 UBZ-type zinc finger.
- Information by UniProt
- 2810024C04Rik antibody
- DNA repair protein rad18 antibody
- E3 ubiquitin-protein ligase RAD18 antibody
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cell using ab186835. Green: GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186835. Blue DAPI for nuclear staining.
All lanes : Anti-RAD18 antibody (ab186835) at 1/500 dilution
Lane 1 : Extracts from K562 cell line
Lane 2 : Extracts from MCF7 cell line
Lane 3 : Extracts from Jurkat cell line
Lane 4 : Extracts from 293T cell line
Lane 5 : Extracts from mouse liver
Predicted band size: 56 kDa
ab186835 has been referenced in 1 publication.
- Wu B et al. High expression of RAD18 in glioma induces radiotherapy resistance via down-regulating P53 expression. Biomed Pharmacother 112:108555 (2019). PubMed: 30798132