Anti-RAD18 antibody (ab188235)
Key features and details
- Rabbit polyclonal to RAD18
- Suitable for: WB, IP, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RAD18 antibody
See all RAD18 primary antibodies -
Description
Rabbit polyclonal to RAD18 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human RAD18 aa 250 to the C-terminus.
Database link: Q9NS91 -
Positive control
- Irradiated U2OS cells and lysate, NIH-3T3 cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000. Predicted molecular weight: 56 kDa.
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IP |
Use at an assay dependent concentration.
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ICC/IF |
1/2000.
Fixation with 3% PFA 10 min, RT and afterwards permeabilization with methanol for 2 min. |
Notes |
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WB
1/2000. Predicted molecular weight: 56 kDa. |
IP
Use at an assay dependent concentration. |
ICC/IF
1/2000. Fixation with 3% PFA 10 min, RT and afterwards permeabilization with methanol for 2 min. |
Target
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Function
E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the RAD18 family.
Contains 1 RING-type zinc finger.
Contains 1 SAP domain.
Contains 1 UBZ-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 56852 Human
- Entrez Gene: 58186 Mouse
- Omim: 605256 Human
- SwissProt: Q9NS91 Human
- SwissProt: Q9QXK2 Mouse
- Unigene: 375684 Human
- Unigene: 103812 Mouse
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Alternative names
- 2810024C04Rik antibody
- DNA repair protein rad18 antibody
- E3 ubiquitin-protein ligase RAD18 antibody
see all
Images
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All lanes : Anti-RAD18 antibody (ab188235) at 1/2000 dilution
Lane 1 : Control U2OS cell lysate
Lane 2 : RAD18 si treated U2OS cell lysate
Lane 3 : NIH-3T3 cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 56 kDa -
Immunofluorescent analysis of U2OS cells fixed in 3% PFA for 10 mins and permeabilised with methanol for 2 mins, then irradiated with 3Gy of IR for 1.5h labeling RAD18 with ab188235 at 1/600 dilution. Rad18 (red) ; DAPI (blue).
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Immunofluorescent analysis of RAD18 expression in U2OS control cells and RAD18 siRNA treated U2OS cells, using ab188235 at 1/2000 dilution.
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Immunofluorescent analysis U2OS cells fixed in 3% PFA 10 mins and permeabilised with methanol 2 mins irradiated with 3Gy IR for 1.5h labeling RAD18 with ab188235 (1), DNA (2) and merged (3)
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Western blot analysis (using ab188235) of immunoprecipitation from U2OS cell lysates(lysated in 0.5% NP40)
Lane 1: Input
Lane 2: immunoprecipitate with control serum+protein A sepharose.
Lane 3: immunoprecipitate with ab188235+ protein A sepharose.
Protocols
Datasheets and documents
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Datasheet download
References (8)
ab188235 has been referenced in 8 publications.
- Wang YC et al. Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA ubiquitination. Cell Rep 42:112296 (2023). WB ; Human . PubMed: 36961817
- Yu R et al. LncRNA CTBP1-DT-encoded microprotein DDUP sustains DNA damage response signalling to trigger dual DNA repair mechanisms. Nucleic Acids Res 50:8060-8079 (2022). WB ; Human . PubMed: 35849344
- Mustofa MK et al. RAD18 mediates DNA double-strand break-induced ubiquitination of chromatin protein. J Biochem N/A:N/A (2021). ICC ; Human . PubMed: 33508099
- Li P et al. RAD18 promotes colorectal cancer metastasis by activating the epithelial-mesenchymal transition pathway. Oncol Rep N/A:N/A (2020). WB, IHC ; Human . PubMed: 32319669
- Xue H et al. Long non-coding RNA LINC00858 aggravates the oncogenic phenotypes of ovarian cancer cells through miR-134-5p/RAD18 signaling. Arch Gynecol Obstet 302:1243-1254 (2020). WB ; Human . PubMed: 32875345
- Spies J et al. 53BP1 nuclear bodies enforce replication timing at under-replicated DNA to limit heritable DNA damage. Nat Cell Biol N/A:N/A (2019). WB, IF ; Human . PubMed: 30804506
- Wu X et al. Crosstalk between Lys63- and Lys11-polyubiquitin signaling at DNA damage sites is driven by Cezanne. Genes Dev N/A:N/A (2019). WB, IF ; Human . PubMed: 31699778
- Meng X et al. Genetic Deficiency of Mtdh Gene in Mice Causes Male Infertility via Impaired Spermatogenesis and Alterations in the Expression of Small Non-coding RNAs. J Biol Chem 290:11853-64 (2015). IHC ; Mouse . PubMed: 25787082