Overview

  • Product name

  • Description

    Rabbit polyclonal to RAD18
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human RAD18 aa 295-495.
    Sequence:

    KSAAEI VREIENIEKT RMRLEASKLN ESVMVFTKDQ TEKEIDEIHS KYRKKHKSEF QLLVDQARKG YKKIAGMSQK TVTITKEDES TEKLSSVCMG QEDNMTSVTN HFSQSKLDSP EELEPDREED SSSCIDIQEV LSSSESDSCN SSSSDIIRDL LEEEEAWEAS HKNDLQDTEI SPRQNRRTRA AESAEIEPRN KRNRN


    Database link: Q9NS91

  • Positive control

    • Irradiated U2OS cells and lysate, NIH-3T3 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab188235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 56 kDa.
IP Use at an assay dependent concentration.
ICC/IF 1/2000.

Fixation with 3% PFA 10 min, RT and afterwards permeabilization with methanol for 2 min.

Target

  • Function

    E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the RAD18 family.
    Contains 1 RING-type zinc finger.
    Contains 1 SAP domain.
    Contains 1 UBZ-type zinc finger.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2810024C04Rik antibody
    • DNA repair protein rad18 antibody
    • E3 ubiquitin-protein ligase RAD18 antibody
    • EC 6.3.2.- antibody
    • hHR 18 antibody
    • hHR18 antibody
    • hRAD 18 antibody
    • hRAD18 antibody
    • MGC156682 antibody
    • Postreplication repair E3 ubiquitin-protein ligase RAD18 antibody
    • Postreplication repair protein hRAD18p antibody
    • Postreplication repair protein RAD18 antibody
    • RAD 18 antibody
    • RAD18 antibody
    • RAD18 homolog (S. cerevisiae) antibody
    • RAD18 homolog antibody
    • RAD18 S. cerevisiae homolog antibody
    • RAD18 S. cerevisiae homolog of antibody
    • RAD18 transcript variant antibody
    • RAD18_HUMAN antibody
    • Rad18sc antibody
    • Radiation sensitivity protein 18 antibody
    • RING finger protein 73 antibody
    • RNF 73 antibody
    • RNF73 antibody
    • Structural maintenance of chromosomes protein 6 antibody
    • YCR066W antibody
    • YCR66W antibody
    see all

Images

  • All lanes : Anti-RAD18 antibody (ab188235) at 1/2000 dilution

    Lane 1 : Control U2OS cell lysate
    Lane 2 : RAD18 si treated U2OS cell lysate
    Lane 3 : NIH-3T3 cell lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 56 kDa

  • Immunofluorescent analysis of U2OS cells fixed in 3% PFA for 10 mins and permeabilised with methanol for 2 mins, then irradiated with 3Gy of IR for 1.5h labeling RAD18 with ab188235 at 1/600 dilution. Rad18 (red) ; DAPI (blue).

  • Immunofluorescent analysis of RAD18 expression in U2OS control cells and RAD18 siRNA treated U2OS cells, using ab188235 at 1/2000 dilution.

  • Immunofluorescent analysis U2OS cells fixed in 3% PFA 10 mins and permeabilised with methanol 2 mins irradiated with 3Gy IR for 1.5h labeling RAD18 with ab188235 (1), DNA (2) and merged (3)

  • Western blot analysis (using ab188235) of immunoprecipitation from U2OS cell lysates(lysated in 0.5% NP40)

    Lane 1: Input

    Lane 2: immunoprecipitate with control serum+protein A sepharose.

    Lane 3: immunoprecipitate with ab188235+ protein A sepharose.

References

This product has been referenced in:

  • Spies J  et al. 53BP1 nuclear bodies enforce replication timing at under-replicated DNA to limit heritable DNA damage. Nat Cell Biol N/A:N/A (2019). WB, IF ; Human . Read more (PubMed: 30804506) »
  • Meng X  et al. Genetic Deficiency of Mtdh Gene in Mice Causes Male Infertility via Impaired Spermatogenesis and Alterations in the Expression of Small Non-coding RNAs. J Biol Chem 290:11853-64 (2015). IHC ; Mouse . Read more (PubMed: 25787082) »
See all 2 Publications for this product

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