Key features and details
- Rabbit polyclonal to RAD18
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RAD18 antibody
See all RAD18 primary antibodies
DescriptionRabbit polyclonal to RAD18
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Human
Does not react with: Mouse
Fusion protein (Human) from the C-terminus with a proprietary tag.
- A549 cells GM637 cells
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab79763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 56 kDa.|
|IP||Use at an assay dependent dilution.|
|ICC/IF||Use at an assay dependent dilution.|
FunctionE3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the RAD18 family.
Contains 1 RING-type zinc finger.
Contains 1 SAP domain.
Contains 1 UBZ-type zinc finger.
- Information by UniProt
- 2810024C04Rik antibody
- DNA repair protein rad18 antibody
- E3 ubiquitin-protein ligase RAD18 antibody
Indirect immunofluorescence staining of GM637 cells . Note staining of nuclei.
Anti-RAD18 antibody (ab79763) at 1/1000 dilution + A549 crude extract
Predicted band size: 56 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
The lower thick band is native RAD18 and the upper thin band is mono-ubiquitinated RAD18 protein.
ab79763 has been referenced in 5 publications.
- Wanzel M et al. CRISPR-Cas9-based target validation for p53-reactivating model compounds. Nat Chem Biol 12:22-8 (2016). WB . PubMed: 26595461
- Peng M et al. Crosstalk between BRCA-Fanconi anemia and mismatch repair pathways prevents MSH2-dependent aberrant DNA damage responses. EMBO J N/A:N/A (2014). WB ; Human . PubMed: 24966277
- Zeman MK et al. DNA damage-specific deubiquitination regulates Rad18 functions to suppress mutagenesis. J Cell Biol 206:183-97 (2014). Human . PubMed: 25023518
- Jones MJ et al. Dysregulation of DNA polymerase ? recruitment to replication forks results in genomic instability. EMBO J 31:908-18 (2012). WB ; Human . PubMed: 22157819
- Cotto-Rios XM et al. APC/CCdh1-dependent proteolysis of USP1 regulates the response to UV-mediated DNA damage. J Cell Biol 194:177-86 (2011). WB ; Human . PubMed: 21768287