• Product name

  • Description

    Rabbit polyclonal to RAD18
  • Host species

  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Fusion protein (Human) from the C-terminus with a proprietary tag.

  • Positive control

    • A549 cells GM637 cells



Our Abpromise guarantee covers the use of ab79763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 56 kDa.
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.


  • Function

    E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the RAD18 family.
    Contains 1 RING-type zinc finger.
    Contains 1 SAP domain.
    Contains 1 UBZ-type zinc finger.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • 2810024C04Rik antibody
    • DNA repair protein rad18 antibody
    • E3 ubiquitin-protein ligase RAD18 antibody
    • EC 6.3.2.- antibody
    • hHR 18 antibody
    • hHR18 antibody
    • hRAD 18 antibody
    • hRAD18 antibody
    • MGC156682 antibody
    • Postreplication repair E3 ubiquitin-protein ligase RAD18 antibody
    • Postreplication repair protein hRAD18p antibody
    • Postreplication repair protein RAD18 antibody
    • RAD 18 antibody
    • RAD18 antibody
    • RAD18 homolog (S. cerevisiae) antibody
    • RAD18 homolog antibody
    • RAD18 S. cerevisiae homolog antibody
    • RAD18 S. cerevisiae homolog of antibody
    • RAD18 transcript variant antibody
    • RAD18_HUMAN antibody
    • Rad18sc antibody
    • Radiation sensitivity protein 18 antibody
    • RING finger protein 73 antibody
    • RNF 73 antibody
    • RNF73 antibody
    • Structural maintenance of chromosomes protein 6 antibody
    • YCR066W antibody
    • YCR66W antibody
    see all


  • Indirect immunofluorescence staining of GM637 cells . Note staining of nuclei.
  • Anti-RAD18 antibody (ab79763) at 1/1000 dilution + A549 crude extract

    Predicted band size: 56 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.

    The lower thick band is native RAD18 and the upper thin band is mono-ubiquitinated RAD18 protein.


This product has been referenced in:

  • Wanzel M  et al. CRISPR-Cas9-based target validation for p53-reactivating model compounds. Nat Chem Biol 12:22-8 (2016). WB . Read more (PubMed: 26595461) »
  • Peng M  et al. Crosstalk between BRCA-Fanconi anemia and mismatch repair pathways prevents MSH2-dependent aberrant DNA damage responses. EMBO J N/A:N/A (2014). WB ; Human . Read more (PubMed: 24966277) »
See all 5 Publications for this product

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